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sPLA2 activity kit

ADI-907-002 96 wells 404.00 USD
Do you need bulk/larger quantities?
  • Higher throughput - assay up to 42 samples in duplicate per 96 well kit
  • Sensitive - detect < 4 U/ml
  • Fast - results in just 40 minutes!
The sPLA2 activity kit is a higher throughput assay for the quantification of up to 42 samples in duplicate per 96 well kit. This kit provides safe, sensitive, and reliable results in under an hour.
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Product Specification

Alternative Name:Secretory phospholipase A2
Sensitivity:3.85 Units/ml (range 5 - 80 Units/ml)
Applications:Colorimetric detection
Activity assay
Application Notes:For the quantitative determination of sPLA2 in culture supernatants, cell lysates, and buffers from any species.
Use/Stability:Store all components +4°, except Substrate at -20°.
Shipping:Shipped on Blue Ice
Contents:Microtiter plate, Standard, Reaction buffer concentrate, Substrate, Stop reagent, Color reagent
Scientific Background:Secretory Phospholipase A2s (sPLA2s) are a group of related enzymes that upon activation, cleave an acyl ester bond in the sn-2 position in glycerophospholipid. These are extracellular enzymes that have high disulfide bond content, low molecular mass (14KDa), and require mM levels of Ca+ for catalysis. sPLA2 enzymes play a crucial role in the generation of arachidonic acid, which is further metabolized to proinflammatory eicosanoids.

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