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hROS, (detection reagent)

 
ADI-906-043-150 150 tests 237.00 USD
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Cell permeable dye for the quantitative detection of highly reactive oxygen species (hROS). Species independent.

Product Specification

Alternative Name:Reactive Oxygen Species
 
Formulation:Liquid. APF (aminophenyl fluorescein) in DMF. Sufficient for ~150 tests.
 
Application Notes:Detect and quantitate hROS via Flow Cytometry cytometry, immunofluorescent microscopy, or fluorescent plate-based assay.
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Technical Info/Product Notes:The hROS detection reagent, aminophenyl fluorescein (APF), is a selective indicator for highly reactive oxygen species (hROS). APF is a cell permeable dye that can be used to detect hydroxyradical, peroxynitrite, and hypochlorite production in cells. This fluorescent reagent has little reactivity towards other ROS such as singlet oxygen, superoxide, hydrogen peroxide, nitric oxide, and alkyl peroxide. APF itself is not very fluorescent, however when reacted with hROS strong dose dependent fluorescence is exhibited. This versatile detection reagent can be adapted for use in quick one-step homogenous high throughput fluorescent plate-based assays, immunofluorescent microscopy, or flow cytometry.
 

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