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α-Actinin monoclonal antibody (7A4)

 
ADI-905-891-100 100 µl 410.00 USD
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Product Specification

Alternative Name:ACTN1
 
Clone:7A4
 
Host:Mouse
 
Isotype:IgG2a
 
Immunogen:Recombinant human α-actinin (aa 650-893).
 
Species reactivity:Human
 
Applications:WB
 
Recommended Dilutions/Conditions:Western Blot (1:100,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~98kDa (full length) by Western blot.
 
Purity Detail:Protein G-affinity purified.
 
Formulation:Liquid. In PBS, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments.
 
905-891 WB
Western blot analysis of HeLa cell lysate transfected with human alpha-actinin gene expression vector, probed with α-Actinin (ACTN1) mAb (7A4) at 1:100,000 dilution.
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905-891 WB

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