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Oct-3/4 monoclonal antibody (9B7)

 
ADI-905-855-100 100 µl 410.00 USD
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Product Specification

Alternative Name:POU5F1, POU domain class 5 transcription factor 1, OTF3/4, Octamer-binding protein 3/4
 
Clone:9B7
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Recombinant human Oct4 (aa 1-360).
 
UniProt ID:Q01860
 
Species reactivity:Human
 
Applications:WB
 
Recommended Dilutions/Conditions:Western Blot (1:5,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~39kDa (full length) by Western blot.
 
Purity Detail:Protein G affinity purified.
 
Formulation:Liquid. In PBS, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Oct4 is a members of Oct family of transcription factors which specifically interact with Octamer motif ATGCAAAT, a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes. All of the members of the Oct family contain two highly conserved domains which are separated by 14-26 variable amino acids. These include the POU homeodomain and the POU-specific domain. Both are required for DNA binding and are involved in protein-protein interactions. Evidences indicate that regulation of Oct family transcription factors occurs at the level of phosphorylation. Oct4 is renowned as a transcription factor expressed by undifferentiated embryonic stem cells and embryonic germ cells.
 
905-855 WB
Western blot analysis of HEK-293T cell lysates transfected with human OCT4 expression vector, probed with Oct-3/4 (POU5F1) mAb (9B7) at 1:5000 dilution.
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905-855 WB

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