Product Details
| Alternative Name: | OPRK1, KOR-1 |
| |
| Host: | Rabbit |
| |
| Immunogen: | Synthetic peptide corresponding to the sequence near the N-terminus of mouse opioid receptor κ. |
| |
| UniProt ID: | P33534 |
| |
| Species reactivity: | Human, Mouse, Rat
|
| |
| Crossreactivity: | Predicted species reactivity based on epitope sequence homology: dog, bovine, horse. |
| |
| Applications: | ELISA, IHC, WB
|
| |
| Application Notes: | Suitable for cell-based and membrane-based ELISA.
Predicted MW of ~43kDa. Higher MW species (~50kDa) and intermediates also observed by Western blot which reflect post-translational modification of the receptor. |
| |
| Purity Detail: | Peptide affinity purified. |
| |
| Formulation: | Liquid. In PBS containing 50% glycerol and 0.01% sodium azide. |
| |
| Handling: | Keep on ice at all times. |
| |
| Shipping: | Blue Ice |
| |
| Long Term Storage: | -20°C |
| |
| Scientific Background: | The opioid receptors delta (DOP, OP1), kappa (KOP, OP2), and mu (MOP, OP3) are members of the seven-transmembrane domain-containing receptor family of G-protein coupled receptors, sharing nearly 60% identity, but varying greatly in the extracellular N-terminus region of the receptors. The delta, kappa and mu-receptors serve as functional receptors for endogenous enkephalin, dynorphin, and beta-endorphin peptide ligands, respectively, mediating their signal via coupling to Gi/o alpha subunits to inhibit adenylyl cyclases and calcium conductance (N- and L-type channels), and to stimulate MAPK activation and potassium conductance. Delta-opioid receptors are highly expressed in the olfactory bulb, neocortex, caudate putamen and nucleus accumbens, with peripheral expression in the gastro-intestinal tract and vas deferens. Delta-opioid receptors function to inhibit neurotransmitter release resulting in a variety of biological effects including analgesia, motor integration, gastro-intestinal motility, olfaction, |
| |
| Regulatory Status: | RUO - Research Use Only |
| |
| Manufacturer: | Manufactured by Proteimax. |
| |
Western blot analysis: MW marker (1) and 50µg rat brain extract (1) probed with Opioid Receptor kappa pAb at 1µg/ml
Membrane ELISA: Lewis rat striatum membranes (5µg/well) were treated with 1 μM concentrations of agonist (Endorphin B) and probed with Opioid Receptor kappa pAb (1:250 to 1:2000 of a 1µg/μl stock solution) by ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean ± SEM (n=4).
Please mouse over
Product Literature References
Generation of G protein-coupled receptor antibodies differentially sensitive to conformational states: A.S. Heimann, et al.; PloS One
12, e0187306 (2017),
Abstract;
Full Text
Related Products
