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Nitrotyrosine monoclonal antibody (7A5)

 
ADI-905-763-100 100 µg 313.00 USD
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Product Specification

Clone:7A5
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:KLH modified with Nitrotyrosine.
 
Species reactivity:Human, Mouse, Rat
Dog
 
Applications:ELISA, WB
 
Recommended Dilutions/Conditions:ELISA (0.05µg/ml)
Western Blot (0.5µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Thiophilic adsorption and size exclusion chromatography purified.
 
Formulation:Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.
 
Reconstitution:Reconstitute with 1ml water (15 minutes at room temperature).
 
Use/Stability:Stable at -80°C up to 1 year, at 4°C up to 3 months.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The enzymatic conversion of arginine to citrulline by nitric oxide synthase (NO Synthase) leads to the formation of nitric oxide (NO). Peroxynitrite is formed following reaction of NO with superoxide, producing a potent oxidant that reacts with a variety of biological molecules, including tyrosine residues in proteins. Tyrosine nitration can inactivate enzymes and receptors that depend on tyrosine residues for their activity. Nitration prevents phosphorylation of tyrosine residues important for signal transduction.
 
905-763 WB
Western blot analysis of Nitrotyrosine-modified BSA probed with Nitrotyrosine mAb (7A5) at 1µg/ml.
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905-763 WB

Product Literature References

EGCG, a green tea catechin, attenuates the progression of heart failure induced by the heart/muscle-specific deletion of MnSOD in mice: J. Oyama, et al.; J. Cardiol. 6, 30109 (2016), Application(s): Analysis of protein expression, frozen heart tissues, Abstract;

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