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[pTyr278]GSK-3α and [pTyr215]GSK-3β monoclonal antibody (6D3)

 
ADI-905-762-100 100 µg 323.00 USD
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Product Specification

Alternative Name:Glycogen synthase kinase-3α/β
 
Clone:6D3
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Synthetic peptide corresponding to a portion of mouse GSK phosphorylated at Tyr215/278.
 
UniProt ID:Q2NL51 (GSK3A), Q9WV60 (GSK3B)
 
Species reactivity:Human, Mouse
 
Applications:WB
 
Purity Detail:Thiophilic adsorption and size exclusion chromatography purified.
 
Formulation:Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.
 
Reconstitution:Reconstitute with 1ml water (15 minutes at room temperature).
 
Use/Stability:Stable at -80°C up to 1 year, at 4°C up to 3 months.
 
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Glycogen Synthase Kinase 3β (GSK-3β) is a unique serine/threonine kinase that is inactivated by phosphorylation. In response to insulin binding, PKB/AKT phosphorylates GSK-3β on serine 9, which prevents GSK-3β from phosphorylating glycogen synthase. Unphosphorylated glycogen synthase is active and able to synthesize glycogen. GSK-3β is also unique in that it requires a substrate that has been phosphorylated by a distinct kinase before it can phosphorylate the substrate. This phosphate priming mechanism explains why phosphorylation of serine 9 inactivates GSK-3β. The phosphorylated serine binds to the GSK-3β priming phosphate position and prevents binding of alternative substrates. In addition to insulin signaling, GSK-3β participates in the Wnt signaling pathway, where it forms a complex with axin, beta-catenin and adenomatous polyposis coli (APC) protein. In the presence of Wnts, GSK-3β is unable to phosphorylate beta-catenin, which leads to stabilization of beta-catenin. The Wnt pathway inactivates GSK-3β via the proteins, Dishevelled and FRAT, which disrupt the interaction of GSK-3β with axin, beta-catenin, and APC. Clinically, there is considerable interest in GSK-3β inhibitors because they may mimic the effect of insulin or reduce the hyperphosphorylation of Tau that is observed in Alzheimer's Disease.
 
905-762 WB
Western blot analysis of serum starved cancer cells: HeLa (1), HepG2 (2), HEK293 (3), SH-SY5Y (4), MDCK (5), PC12 (6), CMT 93 (7), Neuro 2A (8), and NIH-3T3 (9), with GSK3 α/β (phospho-Tyr215/278) mAb (6D3).
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905-762 WB

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