Akt1/2, pAb



ADI-905-755-100	100 µg	200.00 USD

Product Specification

Alternative Name:	PKB, Protein kinase B, RAC

Purity Detail:	Protein A affinity purified.

Formulation:	Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.

Immunogen:	Synthetic peptide corresponding to the C-terminus of human Akt.

Source/Host:	From rabbit.

Specificity:	Recognizes human, mouse and rat Akt1,2. Detects a band of ~56kDa by Western blot.

Application:	Western Blot (1µg/ml, ECL) Optimal conditions must be determined individually for each application.

Long Term Storage:	-20°C

Miscellaneous/General:	The Akt (PKB) family of protein kinases are serine/threonine kinases, with three mammalian family members identified to date (Akt1, Akt2, Akt3). Akt is a well-characterized member of PI3 kinase-mediated signaling pathways, regulating cell growth, apoptosis, glycogen synthesis, and other cellular responses through its phosphorylation of downstream substrates. Akt activation is triggered by binding of phospholipid and phosphorylation at two key residues: Thr308 by PDK1, and Ser473 by PDK2, now identified as mTOR. Deregulation of Akt signaling has been associated with cancer, diabetes, and schizophrenia. Akt1 is the cellular homologue of the murine thymoma retroviral oncogene v-akt, and its role in anti-apoptotic and pro-mitotic pathways have made Akt a molecular target for anti-cancer therapeutic intervention. Akt activation inhibits apoptosis by phosphorylating the Bcl-2 related protein Bad, and increases p53 degradation by phosphorylating mdm2. Mitotic substrates of Akt include GSK-3beta, p21CIP1, and p27KIP1, cell cycle inhibitors negatively regulated by Akt phosphorylation. Akt has been shown to mediate angiogenesis through regulation of thrombospondins, which may cooperate with pro-mitotic and anti-apoptotic functions of Akt to promote tumorigenesis.