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Opioid receptor δ polyclonal antibody

ADI-905-745-100 100 µg 316.00 USD
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Product Specification

Alternative Name:OPRD1, DOR-1
Immunogen:Synthetic peptide corresponding to the N-terminus of mouse opioid receptor δ.
UniProt ID:P32300
Species reactivity:Mouse, Rat
Crossreactivity:Predicted species reactivity based on epitope sequence homology: dog.
Applications:ELISA, IHC, WB
Application Notes:Suitable for cell-based and membrane-based ELISA.
Predicted MW of ~40kDa. Higher MW species (~75kDa) and intermediates also observed by Western blot which may reflect post-translational modification or heterodimerization of the receptor.
Purity Detail:Peptide affinity purified.
Formulation:Liquid. In PBS containing 50% glycerol and 0.01% sodium azide.
Handling:Keep on ice at all times.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:The opioid receptors δ, κ, and μ are members of the seven-transmembrane domain-containing receptor family of G-protein coupled receptors. δ- and μ-receptors serve as functional receptors for endogenous enkephalin and β-endorphin peptide ligands, respectively. δ-opioid receptors are highly expressed in the olfactory bulb, neocortex, caudate putamen and nucleus accumbens, with peripheral expression in the gastro-intestinal tract and vas deferens. δ-receptors inhibit neurotransmitter release resulting in a variety of biological effects including analgesia, motor integration, gastro-intestinal motility, olfaction, respiration, and memory. Neural μ-receptor expression is prominent in the caudate putamen, neocortex, thalamus, nucleus accumbens, hippocampus, and amygdala, as well as in peripheral nervous tissues in the gut. Named for its pharmacological mediation of analgesia by morphine, the μ receptor typically functions as a depressant in physiological processes including respiration, cardiovascular function, gastrointestinal motility, and thermoregulation.
Manufacturer:Manufactured by Proteimax.
905-745 WB
Western blot analysis: MW marker (1) and 50µg rat brain extract (2) probed with Opioid Receptor δ pAb at 1.6µg/ml.
905-745 IHC
Immunohistochemistry analysis: Mouse brain cortex, fixed with 4% paraformaldehyde, stained with Opioid Receptor δ pAb at 1:1000, and visualized at 40x.
905-745 c ELISA
Cellular ELISA: SKNSH cells endogenously expressing Opioid Receptor δ were treated with indicated concentrations of agonist (Deltorphin II) and probed with Opioid Receptor δ pAb (1:500 of a stock solution of 1µg/μL ) by ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean ± SEM (n=3).
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905-745 WB 905-745 IHC 905-745 c ELISA

Product Literature References

Generation of G protein-coupled receptor antibodies differentially sensitive to conformational states: A.S. Heimann, et al.; PloS One 12, e0187306 (2017), Abstract; Full Text

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