Product Details
| Alternative Name: | ADRB2, AR β-2 |
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| Host: | Rabbit |
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| Immunogen: | Synthetic peptide corresponding to the N-terminus of mouse adrenergic receptor β2. |
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| UniProt ID: | P18762 |
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| Species reactivity: | Human, Mouse, Rat
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| Crossreactivity: | Predicted species reactivity based on epitope sequence homology: chimpanzee, orangutan, tamarin monkey, gorilla, rhesus macaque, horse, hamster, guinea pig, wild boar, cat. |
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| Applications: | ELISA, IHC, WB
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| Application Notes: | Suitable for cell-based and membrane-based ELISA.
Predicted MW of ~47kDa. Higher MW species (~65kDa) and intermediates also observed by Western blot which reflect post-translational modification of the receptor. |
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| Purity Detail: | Peptide affinity purified. |
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| Formulation: | Liquid. In PBS containing 50% glycerol and 0.01% sodium azide. |
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| Handling: | Keep on ice at all times. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Adrenergic receptors mediate the hormone and neurotransmitter functions of adrenaline and noradrenaline. Adrenoreceptors function in peripheral tissues and the central nervous system to regulate numerous vascular, metabolic, and neurological functions, and are the target for many therapeutically relevant drugs including those for the treatment of hypertension, asthma, benign prostatic hypertrophy, and depression. Adrenoreceptors are classified by three types and multiple subclasses (alpha1A,1B,1D; alpha2A,2B,2C; and beta1,2,3) based on pharmacological and molecular characteristics. |
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| Regulatory Status: | RUO - Research Use Only |
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| Manufacturer: | Manufactured by Proteimax. |
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Western blot analysis: MW marker (1) and 20µg rat heart membrane extract (2) probed with Adrenergic Receptor (β2) pAb at 2.5µg/ml.
Immunohistochemistry analysis: Fluorescent confocal photomicrograph from rat brainstem. Neurons are labeled with Adrenergic Receptor (β2) pAb and Cy3 conjugated rabbit IgG antibody (H+L).
Membrane ELISA: Lewis rat brain membranes (5µg/well) were treated with indicated concentrations of agonist (Isoproterenol) and antagonist (CGP12177) and probed with Adrenergic Receptor (β2) pAb (1:1000 of a stock solution of 1µg/μL) by ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean ± SEM (n=3).
Cellular ELISA: HEK293 cells expressing Adrenergic Receptor β2 were treated with indicated concentrations of agonist (Isoproterenol) and antagonist (CPG12177) and probed with Adrenergic Receptor (β2) pAb (1:1000 of a stock solution of 1µg/μL ) by ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean ± SEM (n=3).
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Product Literature References
Generation of G protein-coupled receptor antibodies differentially sensitive to conformational states: A.S. Heimann, et al.; PloS One
12, e0187306 (2017),
Abstract;
Full Text
Conformation state-sensitive antibodies to G-protein-coupled receptors: A. Gupta, et al.; J. Biol. Chem.
282, 5116 (2007),
Application(s): ELISA,
Abstract;
Full Text
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