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β-Actin polyclonal antibody

 
ADI-905-733-100 100 µg 356.00 USD
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Product Specification

Alternative Name:ACTA, ACTSA
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to the sequence near the C-terminus of human β-Actin.
 
UniProt ID:P60709
 
Species reactivity:Human, Mouse, Rat
 
Applications:ICC, WB
 
Recommended Dilutions/Conditions:Immunocytochemistry (10µg/ml)
Western Blot (1-2µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~43kDa by Western blot.
 
Purity Detail:Peptide affinity purified.
 
Formulation:Liquid. In PBS containing 0.02% sodium azide.
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Scientific Background:Actin is one of the major proteins of the cytoskeleton. It regulates contractile potential in muscle tissues and helps to control the shape as well as the motility of non-muscle cells. Actin is expressed in all eukaryotic cells. In higher eukaryotes, there are six isoforms of actin.
 
905-733 WB
Western blot analysis of beta-actin in HeLa cell lysate with beta-actin antibody at (A) 0.5, (B) 1 and (C) 2µg/ml.
905-733 ICC
Immunocytochemistry analysis of beta-actin in HeLa cells with beta-actin antibody at 10µg/ml.
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905-733 WB 905-733 ICC

Product Literature References

MicroRNA-27b Enhances the Hepatic Regenerative Properties of Adipose-Derived Mesenchymal Stem Cells: K.D. Chen, et al.; Mol. Ther. Nucleic Acids 5, e285 (2016), Application(s): Western blot, Abstract;
Oxidative stress by layered double hydroxide nanoparticles via an SFK-JNK and p38-NF-κB signaling pathway mediates induction of interleukin-6 and interleukin-8 in human lung epithelial cells: S.J. Choi, et al.; Int. J. Nanomedicine 10, 3217 (2015), Application(s): Western Blot, Abstract; Full Text
Up regulation of Bax and down regulation of Bcl2 during 3-NC mediated apoptosis in human cancer cells: M.H. Naseri, et al.; Cancer Cell Int. 15, 55 (2015), Application(s): Western Blot, Abstract; Full Text

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