Product Details
Alternative Name: | ACTA, ACTSA |
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Host: | Rabbit |
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Immunogen: | Synthetic peptide corresponding to the sequence near the C-terminus of human β-Actin. |
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UniProt ID: | P60709 |
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Species reactivity: | Human, Mouse, Rat
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Applications: | ICC, WB
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Recommended Dilutions/Conditions: | Immunocytochemistry (10µg/ml) Western Blot (1-2µg/ml) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Detects a band of ~43kDa by Western blot. |
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Purity Detail: | Peptide affinity purified. |
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Formulation: | Liquid. In PBS containing 0.02% sodium azide. |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | Actin is one of the major proteins of the cytoskeleton. It regulates contractile potential in muscle tissues and helps to control the shape as well as the motility of non-muscle cells. Actin is expressed in all eukaryotic cells. In higher eukaryotes, there are six isoforms of actin. |
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Regulatory Status: | RUO - Research Use Only |
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Immunocytochemistry analysis of beta-actin in HeLa cells with beta-actin antibody at 10µg/ml.
Western blot analysis of beta-actin in HeLa cell lysate with beta-actin antibody at (A) 0.5, (B) 1 and (C) 2µg/ml.
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Product Literature References
MicroRNA-27b Enhances the Hepatic Regenerative Properties of Adipose-Derived Mesenchymal Stem Cells: K.D. Chen, et al.; Mol. Ther. Nucleic Acids
5, e285 (2016),
Application(s): Western blot,
Abstract;
Oxidative stress by layered double hydroxide nanoparticles via an SFK-JNK and p38-NF-κB signaling pathway mediates induction of interleukin-6 and interleukin-8 in human lung epithelial cells: S.J. Choi, et al.; Int. J. Nanomedicine
10, 3217 (2015),
Application(s): Western Blot,
Abstract;
Full Text
Up regulation of Bax and down regulation of Bcl2 during 3-NC mediated apoptosis in human cancer cells: M.H. Naseri, et al.; Cancer Cell Int.
15, 55 (2015),
Application(s): Western Blot,
Abstract;
Full Text