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Histone H3 (acetyl-Lys9) polyclonal antibody

 
ADI-905-705-100 100 µg 281.00 USD
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Product Specification

Host:Rabbit
 
Immunogen:Synthetic acetyl-peptide corresponding to the N-terminus of mouse histone H3.
 
UniProt ID:P68433 (Histone H3.1), P84228 (Histone H3.2), P84244 (Histone H3.3)
 
Species reactivity:Mouse
 
Crossreactivity:Weakly cross-reacts with human, rat, bovine and dog Histone H3 (acetyl-Lys9).
 
Applications:WB
 
Recommended Dilutions/Conditions:Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Peptide affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The nucleosome is made up of four core histone proteins (H2A, H2B, H3 and H4) and is the primary building block of chromatin. The core histones form an octamer, which is made up of one H3-H4 tetramer and two H2A-H2B dimers. The N-terminal tail of core histones undergoes different post-transcriptional modifications including acetylation, phosphorylation, methylation and ubiquitination. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression.
 
905-705 WB
Western blot analysis of Histone H3 (Acetyl-Lys9) using 3T3 cell extracts treated with TSA. Lane 1: Molecular Weight Markers, Lane 2: 3T3, Lane 3: 3T3 + TSA.
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905-705 WB

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