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mTOR polyclonal antibody

ADI-905-687-100 100 µg 296.00 USD
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Product Specification

Alternative Name:FRAP, FK506 binding protein 12-rapamycin associated protein
Immunogen:Synthetic peptide corresponding to a portion of human mTOR.
UniProt ID:P42345
Source:Purified from rabbit serum.
Species reactivity:Human, Mouse
Recommended Dilutions/Conditions:Western Blot (2µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~289kDa by Western blot.
Purity Detail:Peptide affinity purified.
Formulation:Liquid. In TRIS, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:Mammalian target of rapamycin (mTOR) is an atypical serine-threonine protein kinase and member of the phosphatidylinositol 3-kinase (PI3K) signaling pathway. TORC1 (mTOR, GβL/mLST8 and Raptor) and TORC2 (mTOR, GβL/mLST8, SIN1, and Rictor) complexes control protein synthesis through the direct or indirect phosphorylation of downstream targets such as S6 Kinase and 4EBP1, key regulators of mRNA recruitment to ribosomes. Through its involvement in several key signaling pathways and its critical role in transcription and translation, mTOR mediates cell proliferation, differentiation, and survival. Studies identified the TORC2 complex as the missing kinase activator of Akt/PKB, deregulation of which has been implicated in the pathogenesis of cancer and diabetes.
mTOR polyclonal antibody Western blot
Western blot analysis of MW marker (1), HeLa (2), and 293-EGF (3) cells probed with mTOR pAb.
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mTOR polyclonal antibody Western blot

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