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Caspase-2 monoclonal antibody (4-1-5)

 
ADI-905-685-100 100 µg 291.00 USD
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Product Specification

Clone:4-1-5
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Recombinant full length human Caspase-2.
 
UniProt ID:P42575
 
Species reactivity:Human
 
Applications:WB
 
Recommended Dilutions/Conditions:Western Blot (2µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects bands of ~47kDa (full length) and ~14kDa (cleaved) by Western blot.
 
Purity Detail:Protein G affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The caspases are a family of cysteine proteases that cleave after certain aspartate residues, and are primarily recognized as mediators of apoptosis. caspases are synthesized as inactive zymogens that can be cleaved to form active enzymes following the induction of apoptosis by stress or death receptors. Initiator caspases (e.g. caspase-8 and -10) are activated by dimerization of the zymogen on a dedicated adaptor protein. These activated initiator caspases in-turn cleave downstream effector or executioner caspases (e.g. caspase-3, -6, and -7) in a cascade-like manner, which cleave key cellular proteins that lead to the morphological changes associated with apoptotic cell death.
 
905-685 WB
Western blot analysis of MW marker (1), Jurkat (2) and Jurkat + staurosporine (3) probed with Caspase 2 mAb (4-1-5)
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905-685 WB

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