Alternative Name: | Second mitochondria-derived activator of caspase |
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Clone: | 79-1-83 |
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Host: | Mouse |
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Isotype: | IgG2b |
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Immunogen: | Recombinant full length human Smac/DIABLO. |
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UniProt ID: | Q9NR28 |
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Source: | Purified from mouse ascites. |
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Species reactivity: | Human Monkey
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Applications: | IHC (PS), WB
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Recommended Dilutions/Conditions: | Western Blot (1µg/ml, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Purity Detail: | Protein G affinity purified. |
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Formulation: | Liquid. In PBS, pH 7.2, containing 0.09% sodium azide and 50% glycerol. |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | Smac/DIABLO is one of the pro-apoptotic proteins normally found in the intermembrane space of the mitochondria. In response to apoptotic stimuli, Smac/DIABLO is released into the cytosol resulting in permeabilization of the mitochondrial membrane. Cytosolic Smac/DIABLO activates caspase-9 by binding IAP proteins. Changes in the expression level of Smac/DIABLO have been demonstrated in a number of human cancers including melanoma, ovary, and prostate. |
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Regulatory Status: | RUO - Research Use Only |
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IHC Analysis: Human testis tissue stained with Smac/DIABLO, mAb (79-1-83) at 10µg/ml.
IHC Analysis: Human adrenal tissue stained with Smac/DIABLO, mAb (79-1-83) at 10µg/ml.
Western blot analysis of Smac/Diablo. Lane 1: MW marker, Lane 2: 293, Lane 3: HeLa, Lane 4: Jurkat, Lane 5: Vero.
IHC Analysis: Human tonsil tissue stained with Smac/DIABLO, mAb (79-1-83) at 10µg/ml.