Product Details
Alternative Name: | INSR |
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Clone: | C18C4 |
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Host: | Mouse |
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Isotype: | IgG1 |
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Immunogen: | Recombinant cytoplasmic domain of human Insulin Receptor β. |
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UniProt ID: | P06213 |
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Source: | Purified from ascites. |
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Species reactivity: | Human, Mouse, Rat Bovine, Hamster, Porcine, Rabbit, Sheep
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Applications: | WB
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Recommended Dilutions/Conditions: | Western Blot (1:1,000, colorimetric, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Detects a band of ~98kDa by Western blot. |
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Purity Detail: | Protein G affinity purified. |
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Formulation: | Liquid. In PBS, pH 7.2, containing 0.09% sodium azide and 50% glycerol. |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | The Insulin Receptor (InsR) is a heterodimeric receptor tyrosine kinase with an extracellular alpha-chain, a transmembrane domain and an intracellular beta-chain. InsR is activated upon binding of the peptide hormone insulin, leading to autophosphorylation of tyrosine residues 1146, 1150, and 1151 in the activation loop of the beta-chain. Additional autophosphorylation sites such as tyrosine residues 960, 972, 1316, and 1322 regulate the assembly of signal transduction complexes. |
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Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of ADI-905-683: Lane 1: MW Marker, Lane 2: HeLa lysate, Lane 3: MCF-7 lysate, Lane 4: PC-12 lysate, Lane 5: 3T3 lysate.
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Product Literature References
Liver glucose-6-phosphatase proteins in suckling and weaned grey seal pups: structural similarities to other mammals and relationship to nutrition, insulin signalling and metabolite levels: K.A. Bennett, et al.; J. Comp. Physiol. B
183, 1075 (2013),
Abstract;