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GSK-3β polyclonal antibody

 
ADI-905-679-100 100 µg 301.00 USD
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Product Specification

Alternative Name:Glycogen synthase kinase-3β
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to the sequence near the C-terminus of human GSK-3β.
 
UniProt ID:P49841
 
Species reactivity:Human, Mouse, Rat
Bovine
 
Applications:IHC, WB
 
Application Notes:Detects a band of ~47kDa by Western blot.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Glycogen Synthase Kinase 3β (GSK-3β) is a unique serine/threonine kinase that is inactivated by phosphorylation. In response to insulin binding, PKB/AKT phosphorylates GSK-3β on serine 9, which prevents GSK-3β from phosphorylating glycogen synthase. Unphosphorylated glycogen synthase is active and able to synthesize glycogen. GSK-3β is also unique in that it requires a substrate that has been phosphorylated by a distinct kinase before it can phosphorylate the substrate. This phosphate priming mechanism explains why phosphorylation of serine 9 inactivates GSK-3β. The phosphorylated serine binds to the GSK-3β priming phosphate position and prevents binding of alternative substrates. In addition to insulin signaling, GSK-3β participates in the Wnt signaling pathway, where it forms a complex with axin, beta-catenin and adenomatous polyposis coli (APC) protein. In the presence of Wnts, GSK-3β is unable to phosphorylate beta-catenin, which leads to stabilization of beta-catenin. The Wnt pathway inactivates GSK-3β via the proteins, Dishevelled and FRAT, which disrupt the interaction of GSK-3β with axin, beta-catenin, and APC. Clinically, there is considerable interest in GSK-3β inhibitors because they may mimic the effect of insulin or reduce the hyperphosphorylation of Tau that is observed in Alzheimer's Disease.
 
905-679 WB
Western blot analysis of GSK3β pAb. Lane 1: MW marker; Lane 2: HeLa; Lane 3: Jurkat; Lane 4: 3T3; Lane 5: PC-12; Lane 6: MDBK.
905-679 IHC
Immunohistochemistry analysis of human hepatoma tissue with GSK3β pAb.
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905-679 WB 905-679 IHC

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