Alternative Name: | Signal transducer and activator of transcription 5A/B |
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Clone: | 5G4 |
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Host: | Mouse |
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Isotype: | IgG1 |
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Immunogen: | Synthetic peptide corresponding to a portion of Stat5A/B phosphorylated at Tyr695/699. |
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UniProt ID: | P42229 (STAT5A), P51692 (STAT5B) |
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Species reactivity: | Human
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Applications: | ELISA, ICC, IHC, WB
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Recommended Dilutions/Conditions: | ELISA (0.05µg/ml) Immunohistochemistry (1:50) Western Blot (0.5µg/ml, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Purity Detail: | Thiophilic adsorption and size exclusion chromatography purified. |
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Formulation: | Liquid. In PBS containing 50% glycerol, PEG, sucrose, and 0.09% sodium azide. |
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Use/Stability: | Stable at -20°C up to 1 year. |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | Stats (Signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, Stats enter the nucleus to regulate transcription of many different genes. |
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Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of untreated (1) and pervanadate treated (2) MCF-7 cells, probed with Stat5A/B (pTyr694/699) mAb (5G4).
Immunohistochemistry analysis of human hepatoma tissue with Stat5A/B (pTyr694/699) mAb (5G4).
Immunofluorescent analysis (confocal) staining of MCF-7 cells using Stat5A/B (pTyr694/699) mAb (5G4) (green); nuclei are stained in blue pseudocolor using DRAQ5.