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[pTyr695/Tyr699]Stat5 A/B monoclonal antibody (5G4)

 
ADI-905-673-100 100 µg 360.00 USD
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Product Specification

Alternative Name:Signal transducer and activator of transcription 5A/B
 
Clone:5G4
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Synthetic peptide corresponding to a portion of Stat5A/B phosphorylated at Tyr695/699.
 
UniProt ID:P42229 (STAT5A), P51692 (STAT5B)
 
Species reactivity:Human
 
Applications:ELISA, ICC, IHC, WB
 
Recommended Dilutions/Conditions:ELISA (0.05µg/ml)
Immunohistochemistry (1:50)
Western Blot (0.5µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Purity Detail:Thiophilic adsorption and size exclusion chromatography purified.
 
Formulation:Liquid. In PBS containing 50% glycerol, PEG, sucrose, and 0.09% sodium azide.
 
Use/Stability:Stable at -20°C up to 1 year.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Stats (Signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, Stats enter the nucleus to regulate transcription of many different genes.
 
905-673 WB
Western blot analysis of untreated (1) and pervanadate treated (2) MCF-7 cells, probed with Stat5A/B (pTyr694/699) mAb (5G4).
905-673 IF
Immunofluorescent analysis (confocal) staining of MCF-7 cells using Stat5A/B (pTyr694/699) mAb (5G4) (green); nuclei are stained in blue pseudocolor using DRAQ5.
905-673 IHC
Immunohistochemistry analysis of human hepatoma tissue with Stat5A/B (pTyr694/699) mAb (5G4).
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905-673 WB 905-673 IF 905-673 IHC

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