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Shc monoclonal antibody (11F6)

 
ADI-905-665-100 100 µg 366.00 USD
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Product Specification

Alternative Name:Src homology 2 domain containing
 
Clone:11F6
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Synthetic peptide corresponding to the sequence near the C-terminus of Shc.
 
UniProt ID:P29353
 
Species reactivity:Human, Mouse
 
Applications:ELISA, ICC, IHC, WB
 
Recommended Dilutions/Conditions:Immunohistochemistry (1:50)
Western Blot (1µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects bands of ~46kDa, ~52kDa and ~66kDa by Western blot.
 
Purity Detail:Thiophilic adsorption and size exclusion chromatography purified.
 
Formulation:Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.
 
Reconstitution:Reconstitute with 1ml water (15 minutes at room temperature).
 
Use/Stability:Stable at -80°C up to 1 year, at 4°C up to 3 months.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The Src homology 2 domain containing (Shc) adaptor protein links growth factor, cytokine, and antigen receptor stimulation to the Ras signaling pathway, and plays a critical role in insulin signaling, tumorigenesis, and hematopoietic cell growth and differentiation. Structurally, Shc is composed of an N-terminal phosphotyrosine binding (PTB) domain, a medial CH1 domain, and a C-terminal SH2 domain. Upon binding to activate cell-surface receptors, Shc is phosphorylated at three tyrosine residues (Tyr239, Tyr240, Tyr317) triggering recognition of Shc by SH2-containing proteins such as Grb2.
 
905-665 WB
Western blot analysis of cancer cell lysates with Shc mAb (11F6); HeLa (1), HepG2 (2), HEK293 (3), SH-SY5Y (4), MDCK (5), PC12 (6), CMT93 (7), Neuro 2A (8), NIH-3T3 (9).
905-665 IF
Immunofluorescent analysis (confocal) staining of V79 cells using Shc mAb (11F6) (green); nuclei are stained in blue pseudocolor using DRAQ5.
905-665 IHC
Immunohistochemistry analysis of human hepatoma tissue with Shc mAb (11F6).
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905-665 WB 905-665 IF 905-665 IHC

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