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MKK7 monoclonal antibody (10F7)

 
ADI-905-655-100 100 µg 378.00 USD
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Product Specification

Alternative Name:MAP2K7, MAP kinase kinase 7
 
Clone:10F7
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Synthetic peptide corresponding to the sequence near the amino terminus of MKK7 (MAP2K7) conjugated to hemocyanin.
 
UniProt ID:O14733
 
Species reactivity:Human, Mouse, Rat
Dog
 
Applications:ELISA, ICC, IHC, WB
 
Recommended Dilutions/Conditions:ELISA (0.1µg/ml)
Immunohistochemistry (1:50)
Western Blot (0.5µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~45kDa by Western blot.
 
Purity Detail:Thiophilic adsorption and size exclusion chromatography purified.
 
Formulation:Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.
 
Reconstitution:Reconstitute with 1ml water (15 minutes at room temperature).
 
Use/Stability:Stable at -80°C up to 1 year, at 4°C up to 3 months.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Members of the MAP kinase family of protein kinases (e.g. p38 and Jnk) are subject to dual phosphorylation of a conserved T-X-Y motif in their activation domain by upstream members of the MAP/ERK kinase (MEK/MKK) family. MKK3 and MKK6 phosphorylate p38 in response to cytokine stimulation and cellular stress, while MKK7 and MKK4 activate the Jnk kinases by phosphorylation of the threonine and tyrosine residues, respectively.
 
905-655 WB
Western blot analysis of serum starved cancer cells with MKK7 (MAP2K7) mAb (10F7); A431 (1), A549 (2), SK0V3 (3), OVCAR5 (4), HaCaT (5), PC3 (6), HeLa (7), HepG2 (8).
905-655 IF
Immunofluorescent analysis (confocal) staining of Caco2 cells using MKK7 (MAP2K7) mAb (10F7) (green); nuclei are stained in blue pseudocolor using DRAQ5.
905-655 IHC
Immunohistochemistry analysis of human lung cancer tissue with MKK7 (MAP2K7) mAb (10F7).
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905-655 WB 905-655 IF 905-655 IHC

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