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Jnk3 monoclonal antibody (4G6)

 
ADI-905-650-100 100 µg 399.00 USD
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Product Specification

Alternative Name:c-Jun N-terminal kinase 3, SAP kinase 1b, SAPK1b, MAP kinase 10, MAPK10
 
Clone:4G6
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Synthetic peptide corresponding to the sequence near the N-terminus of Jnk3.
 
UniProt ID:P53779
 
Species reactivity:Human
 
Applications:IHC, WB
 
Recommended Dilutions/Conditions:Immunohistochemistry (1:50)
Western Blot (0.5µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~54kDa by Western blot.
 
Purity Detail:Thiophilic adsorption and size exclusion chromatography purified.
 
Formulation:Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.
 
Reconstitution:Reconstitute with 1ml water (15 minutes at room temperature).
 
Use/Stability:Stable at -80°C up to 1 year, at 4°C up to 3 months.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The Stress-activated protein kinases alpha and gamma (SAPKalpha and gamma), also known as c-Jun NH2-terminal kinases 1 and 2 (Jnk1/2) are serine/ threonine protein kinases belonging to the MAP kinase family. The extracellular stimuli leading to cell stress include inflammatory cytokines (via Rho and Rac), growth factors, protein synthesis inhibitors and osmotic stress amongst others. Both Jnk1 and Jnk2 must be dually phosphorylated in the activation loop, at threonine 183 and tyrosine 185, to achieve full enzymatic activity.
 
905-650 WB
Western blot analysis of serum starved cancer cell A431 (1), A549 (2), SKOV3 (3), OVCAR5 (4), HaCaT (5), PC3 (6), HeLa (7), and HepG2 (8), probed with Jnk3 mAb (4G6).
905-650 IHC
Immunohistochemistry analysis of human hepatoma tissue with Jnk3 mAb (4G6).
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905-650 WB 905-650 IHC

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