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Fos monoclonal antibody (8B5)

 
ADI-905-641-100 100 µg 410.00 USD
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Product Specification

Clone:8B5
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Synthetic peptide corresponding to a portion of human c-Fos .
 
UniProt ID:P01100
 
Species reactivity:Human, Mouse, Rat
Dog
 
Applications:ELISA, IHC, WB
 
Recommended Dilutions/Conditions:ELISA (0.1µg/ml)
Immunohistochemistry (1:50)
Western Blot (0.5µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~50kDa by Western blot.
 
Purity Detail:Thiophilic adsorption and size exclusion chromatography purified.
 
Formulation:Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.
 
Reconstitution:Reconstitute with 1ml water (15 minutes at room temperature).
 
Use/Stability:Stable at -80°C up to 1 year, at 4°C up to 3 months.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The immediate early gene product c-Fos is expressed following mitogenic stimulation and functions as a sensor for MAPK signal duration. When MAPK activation is sustained, c-Fos is phosphorylated by MAPK at Ser374. Phosphorylation stabilizes the c-Fos protein and primes c-Fos for additional phosphorylation at Thr325. During transient MAPK activation, activity declines before accumulation of the c-Fos protein.
 
905-641 WB
Western blot analysis of serum -starved cancer cells A431 (1), A549 (2), SKOV3 (3), OVCAR5 (4), HaCaT (5), PC3 (6), HeLa (7) and HepG2 (8), probed with c-Fos mAb (8B5).
905-641 IHC
Immunohistochemistry analysis of human hepatoma tissue with c-Fos mAb (8B5).
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905-641 WB 905-641 IHC

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