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[pSer374]Fos monoclonal antibody (34E4)

 
ADI-905-640-100 100 µg 399.00 USD
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Product Specification

Clone:34E4
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Synthetic peptide corresponding to a portion of human c-Fos phosphorylated at Ser374.
 
UniProt ID:P01100
 
Species reactivity:Human
Dog
 
Applications:ELISA, IHC, WB
 
Recommended Dilutions/Conditions:ELISA (0.1µg/ml)
Immunohistochemistry (1:50)
Western Blot (0.5µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~50kDa by Western blot.
 
Purity Detail:Thiophilic adsorption and size exclusion chromatography purified.
 
Formulation:Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.
 
Reconstitution:Reconstitute with 1ml water (15 minutes at room temperature).
 
Use/Stability:Stable at -80°C up to 1 year, at 4°C up to 3 months.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The immediate early gene product c-Fos is expressed following mitogenic stimulation and functions as a sensor for MAPK signal duration. When MAPK activation is sustained, c-Fos is phosphorylated by MAPK at Ser374. Phosphorylation stabilizes the c-Fos protein and primes c-Fos for additional phosphorylation at Thr325. During transient MAPK activation, activity declines before accumulation of the c-Fos protein.
 
905-640 WB
Western blot analysis of control (1) and serum -starved cancer cells treated with 10ng/ml EGF for 5 min (2), 15 min (3), 30 min (4), 1 hr (5), 2 hrs (6), 4 hrs (7) and 8 hrs (9), probed with c -Fos (pSer374) mAb (34E4).
905-640 IHC
Immunohistochemistry analysis of human hepatoma tissue with phospho c-Fos mAb (34E4).
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905-640 WB 905-640 IHC

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