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[pSer133]CREB monoclonal antibody (10E9)

 
ADI-905-639-100 100 µg 378.00 USD
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Product Specification

Alternative Name:cAMP responsive element binding protein
 
Clone:10E9
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Synthetic peptide corresponding to a portion of CREB phosphorylated at Ser133.
 
UniProt ID:P16220 (human)
 
Species reactivity:Human, Mouse
Dog
 
Applications:ELISA, WB
 
Recommended Dilutions/Conditions:ELISA (0.05µg/ml)
Western Blot (0.5µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects bands of ~43kDa (CREB) and ~38kDa (ATF1) by Western blot.
 
Purity Detail:Thiophilic adsorption and size exclusion chromatography purified.
 
Formulation:Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.
 
Reconstitution:Reconstitute with 1ml water (15 minutes at room temperature).
 
Use/Stability:Stable at -80°C up to 1 year, at 4°C up to 3 months.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:The transcription factor CREB (cAMP response element binding) binds the palindromic octanucleotide TGACGTCA. Members of the ATF/CREB family include CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2, and ATF-3. Phosphorylation of CREB allows recruitment of CREB-binding protein (CBP), a large co-activator that contacts the general transcriptional machinery. CREB-mediated transcription regulates diverse cellular responses, including intermediary metabolism, neuronal signaling, cell proliferation, and apoptosis.
 
905-639 WB
Western blot analysis of untreated (1), EGF-stimulated (2), or pervanadate-treated (3) A549 cancer cell lysates probed with CREB (pSer133) mAb (10E9).
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905-639 WB

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