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Bad monoclonal antibody (5E6)

 
ADI-905-638-100 100 µg 452.00 USD
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Replaces Prod. #: ALX-804-384

Product Specification

Alternative Name:BCL2 antagonist of cell death
 
Clone:5E6
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Recombinant human Bad.
 
UniProt ID:Q92934
 
Species reactivity:Human
 
Applications:ICC, IHC, IP, WB
 
Recommended Dilutions/Conditions:Immunohistochemistry (1:50)
Immunoprecipitation (1-2µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~19kDa by Western blot.
 
Purity Detail:Thiophilic adsorption and size exclusion chromatography purified.
 
Formulation:Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.
 
Reconstitution:Reconstitute with 1ml water (15 minutes at room temperature).
 
Use/Stability:Stable at -80°C up to 1 year, at 4°C up to 3 months.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Bad is an ~22 kDa pro-apoptotic protein that aids in the initiation of cell death. It exhibits sequence homology to Bcl-2, a protein that prevents apoptosis, within its BH1 and BH2 domains. Bad is phosphorylated at several sites including Ser112, Ser136; phosphorylation by kinases like Akt is known to alter the effects of Bad. Bad has been demonstrated to be phosphorylated at Ser170.
 
Bad monoclonal antibody (5E6) Western blot
Western blot analysis: Recombinant Bad (10ng/lane) was applied to an SDS-PAGE and transferred to a PVDF membrane. The immunoBlot was probed with Bad, mAb (5E6).
Bad monoclonal antibody (5E6) Immunofluorescence
Immunofluorescent analysis (confocal) staining of MCF-7 cells using BAD mAb (5E6) (green); nuclei are stained in blue pseudocolor using DRAQ5.
Bad monoclonal antibody (5E6) Immunohistochemistry
Immunohistochemistry analysis of human breast cancer tissue with BAD mAb (5E6).
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Bad monoclonal antibody (5E6) Western blot Bad monoclonal antibody (5E6) Immunofluorescence Bad monoclonal antibody (5E6) Immunohistochemistry

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