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Bad monoclonal antibody (5E6)

 
ADI-905-638-100 100 µg 430.00 USD
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Replaces Prod. #: ALX-804-384

Product Specification

Alternative Name:BCL2 antagonist of cell death
 
Clone:5E6
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Recombinant human Bad.
 
UniProt ID:Q92934
 
Species reactivity:Human
 
Applications:ICC, IHC, IP, WB
 
Recommended Dilutions/Conditions:Immunohistochemistry (1:50)
Immunoprecipitation (1-2µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~19kDa by Western blot.
 
Purity Detail:Thiophilic adsorption and size exclusion chromatography purified.
 
Formulation:Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose.
 
Reconstitution:Reconstitute with 1ml water (15 minutes at room temperature).
 
Use/Stability:Stable at -80°C up to 1 year, at 4°C up to 3 months.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Bad is an ~22 kDa pro-apoptotic protein that aids in the initiation of cell death. It exhibits sequence homology to Bcl-2, a protein that prevents apoptosis, within its BH1 and BH2 domains. Bad is phosphorylated at several sites including Ser112, Ser136; phosphorylation by kinases like Akt is known to alter the effects of Bad. Bad has been demonstrated to be phosphorylated at Ser170.
 
905-638 WB
Western blot analysis: Recombinant Bad (10ng/lane) was applied to an SDS-PAGE and transferred to a PVDF membrane. The immunoBlot was probed with Bad, mAb (5E6).
905-638 IF
Immunofluorescent analysis (confocal) staining of MCF-7 cells using BAD mAb (5E6) (green); nuclei are stained in blue pseudocolor using DRAQ5.
905-638 IHC
Immunohistochemistry analysis of human breast cancer tissue with BAD mAb (5E6).
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905-638 WB 905-638 IF 905-638 IHC

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