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Osteopontin monoclonal antibody (53)

ADI-905-629-100 100 µg 307.00 USD
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Product Specification

Alternative Name:OPN, SPP1, Secreted phosphoprotein 1, Bone sialoprotein 1
Immunogen:Recombinant human Osteopontin.
UniProt ID:P10451
Species reactivity:Human
Applications:ELISA, IHC (FS), IHC (PS), WB
Purity Detail:Protein G affinity purified.
Formulation:Liquid. In PBS containing 0.1% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Scientific Background:Osteopontin (OPN) is an acidic extracellular matrix cell adhesion protein that is relatively abundant not only in bone matrix, plasma, urine, and milk, but is also found in malignant and atherogenic tissues. Phosphorylation, glycosylation and calcium modifications allow intact and fragmented Osteopontin to direct a variety of diverse responses including tissue remodeling, inflammation and cell survival. Plasma Osteopontin has been shown to provide prognostic information in breast, prostate, colon, and lung cancers as well as metastatic carcinomas. The notable presence of Osteopontin in a variety of tumors is strongly correlated to pathological stage, suggesting its critical role in tumor invasiveness, progression and metastasis. In addition, Osteopontin inhibits inducible nitric oxide synthase activity, thereby protecting tumor cells from NO-mediated macrophage cytotoxic attack. Osteopontin is found in atherosclerotic plaques and may drive a number of diabetic vascular pathologies.
905-629 WB
Western blot analysis: Protein samples (MDA-MB-435 cell lysates, conditioned medium from MDA-MB-435 cells containing secreted OPN or recombinant protein) were resolved by SDS-PAGE on a 8-16% gradient gel, transferred to nitrocellulose, and blocked for 2 hrs in Tris-buffered saline containing 0.05% Tween 20 (TBS-T) and 5% milk powder. For immunodetection, Blots were incubated with anti-OPN 53 (5µg/ml) overnight at 4°C, followed by incubation with a HRP-conjugated goat anti-mouse secondary antibody (in TBS-T) for 1 hr at room temperature. Protein bands were visualized by enhanced chemiluminescence on Kodak X-OMAT film. M= Magic Mark.
905-629 IHC
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded breast cancer tissue stained with 2µg/ml anti-OPN 53.
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905-629 WB 905-629 IHC

Product Literature References

Increased cerebrospinal fluid osteopontin levels and its involvement in macrophage infiltration in neuromyelitis optica: Y. Kariya, et al.; BBA Clin. 3, 126 (2015), Application(s): Western Blotting, cell staining, Abstract; Full Text

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