uNOS, pAb

Alternative Name:	universal NOS, nitric oxide synthase

Purity Detail:	Affinity purified.

Formulation:	Liquid. In PBS, pH 7.4, containing BSA and sodium azide.

Immunogen:	Synthetic peptide corresponding to the sequence near the C-terminus of mouse iNOS and nNOS.

Source/Host:	From rabbit.

Specificity:	Recognizes human, mouse, rat, bovine and pig uNOS. Detects bands of ~155kDa (eNOS), ~130kDa (iNOS), and ~135kDa (nNOS) by Western blot.

Application:	Immunohistochemistry (1:50) Optimal conditions must be determined individually for each application.

Long Term Storage:	+4°C

Miscellaneous/General:	Nitric oxide (NO) is synthesized from L-arginine, oxygen, and NADPH by three known isoforms of heme-containing flavoproteins termed NO synthases (NOS I-III). Analysis of cDNA clones has identified three distinct NOS genes in mammals: neuronal (nNOS/NOS-I), endothelial (eNOS/NOS-III), and inducible (iNOS/NOS-II). eNOS and nNOS are constitutively expressed, agonist-triggered, Ca2+/calmodulin-dependent, and are inhibited by L-arginine analogues (L-NNA, L-NMMA). They are expressed in endothelium, adrenal glands, brain, and platelets. iNOS is inducible, Ca2+/calmodulin-independent, and also inhibited by NMMA and L-NNA. It is expressed in macrophages, hepatocytes, tumor cells, vascular smooth muscle and endothelial cells.

Background / Technical Information:	Entrez Gene ID: 4843/4846