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Smac/DIABLO (CT) polyclonal antibody

ADI-905-244-100 100 µg 374.00 USD
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Product Specification

Alternative Name:Second mitochondria-derived activator of caspase
Immunogen:Synthetic peptide corresponding to the sequence near the C-terminus of human Smac/DIABLO.
UniProt ID:Q9NR28
Species reactivity:Human, Mouse, Rat
Applications:IHC, WB
Recommended Dilutions/Conditions:Immunohistochemistry (5µg/ml)
Western Blot (0.5-1.0µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~25kDa by Western blot.
Purity Detail:Peptide affinity purified.
Formulation:Liquid. In PBS containing 0.02% sodium azide.
Shipping:Blue Ice Not Frozen
Long Term Storage:+4°C
Scientific Background:Smac/DIABLO is one of the pro-apoptotic proteins normally found in the intermembrane space of the mitochondria. In response to apoptotic stimuli, Smac/DIABLO is released into the cytosol resulting in permeabilization of the mitochondrial membrane. Cytosolic Smac/DIABLO activates caspase-9 by binding IAP proteins. Changes in the expression level of Smac/DIABLO have been demonstrated in a number of human cancers including melanoma, ovary, and prostate.
905-244 WB
Western blot analysis of Smac in human heart tissue lysate in the absence (A) or presence (B) of blocking peptide with Smac antibody at 1µg /ml.
905-244 IHC
Immunohistochemistry analysis of Smac/DIABLO in human ovary tissue with Smac antibody at 5µg/ml.
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905-244 WB 905-244 IHC

Product Literature References

Cardiolipin or MTCH2 can serve as tBID receptors during apoptosis: E. Raemy, et al.; Cell Death Differ. 23, 1165 (2016), Application(s): Western blot, Abstract;

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