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RIP3 polyclonal antibody

ADI-905-242-100 100 µg 363.00 USD
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Product Specification

Alternative Name:Receptor-interacting serine/threonine protein kinase 3, RIP-like protein kinase 3, Receptor-interacting protein 3
Immunogen:Synthetic peptide corresponding to a portion of mouse RIP3.
UniProt ID:Q9QZL0
Species reactivity:Mouse, Rat
Applications:IHC (PS), WB
Recommended Dilutions/Conditions:Immunohistochemistry (paraffin sections, 5µg/ml)
Western Blot (0.5-1.0µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~57kDa by Western blot.
Purity Detail:Peptide affinity purified.
Formulation:Liquid. In PBS containing 0.02% sodium azide.
Shipping:Shipped on Blue Ice
Short Term Storage:+4°C
Long Term Storage:-20°C
Scientific Background:A member in the RIP kinase family was identified and designated RIP3 (Receptor-interacting serine/threonine protein kinase-3). RIP3 contains an N-terminal kinase domain but, unlike RIP or RIP2, lacks the C-terminal death or CARD domain. RIP3 binds to RIP and TNF-R1, mediates TNF-R1 induced apoptosis, and attenuates RIP and TNF-R1 induced NF-κB activation.
905-242 WB
Western blot analysis of RIP3 in mouse 3T3 whole cell lysate in the absence (A) or presence (B) of blocking peptide with RIP3 antibody at 1µg/ml.
905-242 IHC
Immunohistochemistry analysis of RIP3 in rat kidney tissue with RIP3 antibody at 5µg/ml.
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905-242 WB 905-242 IHC

Product Literature References

NEMO Prevents RIP Kinase 1-Mediated Epithelial Cell Death and Chronic Intestinal Inflammation by NF-κB-Dependent and -Independent Functions: K. Vlantis, et al.; Immunity 44, 553 (2016), Application(s): Immunoblotting, Abstract;
NEMO regulates a cell death switch in TNF signaling by inhibiting recruitment of RIPK3 to the cell death-inducing complex II: A. Pescatore, et al.; Cell Death Dis. 7, e2346 (2016), Application(s): Cell stimulation and immunoprecipitation, MEF cells, Abstract; Full Text

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