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PUMA (NT) polyclonal antibody

 
ADI-905-237-100 100 µg 356.00 USD
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Product Specification

Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to the sequence near the N-terminus of human PUMAα.
 
UniProt ID:Q9BXH1
 
Species reactivity:Human
 
Applications:IHC, WB
 
Recommended Dilutions/Conditions:Immunohistochemistry (10µg/ml)
Western Blot (2-4µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~23kDa by Western blot.
 
Purity Detail:Peptide affinity purified.
 
Formulation:Liquid. In PBS containing 0.02% sodium azide.
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:-20°C
 
Scientific Background:PUMA, the p53 upregulated modulator of apoptosis gene, encodes for two BH3 domain-containing proteins referred to PUMAa and PUMAb1. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce Cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
 
905-237 WB
Western blot analysis of PUMA expression in K562 cell lysate with PUMA antibody at 2µg /ml.
905-237 IHC
Immunohistochemistry analysis of PUMA in human breast carcinoma with PUMA antibody at 10µg/ml.
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905-237 WB 905-237 IHC

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