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DFF40/CAD (IN) polyclonal antibody

 
ADI-905-201-100 100 µg 347.00 USD
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Product Specification

Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to a portion of human DFF40.
 
UniProt ID:O76075
 
Species reactivity:Human, Mouse, Rat
 
Applications:ICC, WB
 
Recommended Dilutions/Conditions:Immunocytochemistry (10µg/ml)
Western Blot (1µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~40kDa by Western blot.
 
Purity Detail:Peptide affinity purified.
 
Formulation:Liquid. In PBS containing 0.02% sodium azide.
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Scientific Background:Human DNA fragmentation fragment (DFF) 45 and its mouse homologue caspase activated deoxyribonuclease (ICAD) are the inhibitors of DFF40 and CAD, respectively. DFF is composed of two subunits, DFF40/CAD and DFF45/ICAD. Cleavage of DFF45/ICAD, which is mediated by caspase-3, leads to DFF40/CADs activation as a nuclease. Activation of DFF40/CAD, which causes DNA degradation, is the hallmark of apoptotic cell death. DFF40 mRNA is expressed in a limited number of human tissues; pancreas, spleen, prostate, and ovary.
 
905-201 WB
Western blot analysis of DFF40 in Jurkat cell lysate with DFF40 antibody at (A) 0.5, (B) 1 and (C) 2µg/ml.
905-201 ICC
Immunocytochemistry analysis of DFF40 in K562 cells with DFF40 antibody at 10µg/ml.
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905-201 WB 905-201 ICC

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