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Bmf (NT) polyclonal antibody

 
ADI-905-184-100 100 µg 366.00 USD
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Product Specification

Alternative Name:Bcl-2-modifying factor
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to the sequence near the N-terminus of human Bmf.
 
UniProt ID:Q96LC9
 
Species reactivity:Human, Mouse
 
Applications:ICC, WB
 
Recommended Dilutions/Conditions:Western Blot (2µg/ml, HepG2 or 293 cell lysates can be used for positive control)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~25kDa by Western blot.
 
Purity Detail:Peptide affinity purified.
 
Formulation:Liquid. In PBS containing 0.02% sodium azide.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Bmf (Bcl-2 modifying factor) is a member of the Bcl-2 family of apoptotic proteins. The BH3 domain in Bmf is required both for binding to Bcl-2 proteins and for triggering apoptosis. In healthy cells, Bmf associates with the Dynein light chain 2 (DLC2), a component of the myosin V motors, and is sequestered by the cell's actin cytoskeleton. Disruption of the actin cytoskeleton triggers release and activation of Bmf, initiating the downstream apoptotic program.
 
905-184 WB
Western blot analysis of Bmf expression in human HepG2 (lane 1) and 293 (lane 2) cell lysates probed with Bmf antibody at 2µg/ml.
905-184 ICC
Immunocytochemistry analysis of Bmf in HeLa cells with Bmf antibody at 10µg/ml.
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905-184 WB 905-184 ICC

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