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Bmf (NT) polyclonal antibody

 
ADI-905-184-100 100 µg 384.00 USD
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Product Specification

Alternative Name:Bcl-2-modifying factor
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to the sequence near the N-terminus of human Bmf.
 
UniProt ID:Q96LC9
 
Species reactivity:Human, Mouse
 
Applications:ICC, WB
 
Recommended Dilutions/Conditions:Western Blot (2µg/ml, HepG2 or 293 cell lysates can be used for positive control)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~25kDa by Western blot.
 
Purity Detail:Peptide affinity purified.
 
Formulation:Liquid. In PBS containing 0.02% sodium azide.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Bmf (Bcl-2 modifying factor) is a member of the Bcl-2 family of apoptotic proteins. The BH3 domain in Bmf is required both for binding to Bcl-2 proteins and for triggering apoptosis. In healthy cells, Bmf associates with the Dynein light chain 2 (DLC2), a component of the myosin V motors, and is sequestered by the cell's actin cytoskeleton. Disruption of the actin cytoskeleton triggers release and activation of Bmf, initiating the downstream apoptotic program.
 
Bmf (NT) polyclonal antibody Immunocytochemistry
Immunocytochemistry analysis of Bmf in HeLa cells with Bmf antibody at 10µg/ml.
Bmf (NT) polyclonal antibody Western blot
Western blot analysis of Bmf expression in human HepG2 (lane 1) and 293 (lane 2) cell lysates probed with Bmf antibody at 2µg/ml.
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Bmf (NT) polyclonal antibody Immunocytochemistry Bmf (NT) polyclonal antibody Western blot

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