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Apaf-1 (CT) polyclonal antibody

 
ADI-905-179-100 100 µg 356.00 USD
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Product Specification

Alternative Name:APIP
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to the sequence near the C-terminus of human Apaf1. The sequence differs from that of mouse Apaf1 by one amino acid.
 
UniProt ID:O14727
 
Species reactivity:Human, Mouse, Rat
 
Applications:IHC, WB
 
Recommended Dilutions/Conditions:Western Blot (1:500-1:2,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~130kDa by Western blot.
 
Purity Detail:Peptide affinity purified.
 
Formulation:Liquid. In PBS containing 0.02% sodium azide.
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Scientific Background:Apaf-1 (Apoptosis protease-activating factor 1), a mammalian protein homologous to C. elegans CED-4, is an essential adaptor molecule and a key linker of the mitochondrial apoptotic pathway. Apaf-1 binds to Cytochrome c (Apaf-2) leading to the formation of a large multimeric complex. This complex recruits (through CARD) Procaspase-9 and once activated, caspase-9 (Apaf-3) dissociates. Activated caspase-9 in turn cleaves and activates caspase-3, one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf-1 can also associate with caspase-4, caspase-8 and Diva. Apaf-1 is ubiquitously expressed in human tissues.
 
905-179 WB
Western blot analysis of Apaf1 in human heart tissue lysate with Apaf1 antibody at 1µg/ml dilution in the absence (A) or presence (B) of blocking peptide.
905-179 IHC
Immunohistochemistry analysis of Apaf1 in human skin tissue with Apaf1 antibody at 20µg/ml.
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905-179 WB 905-179 IHC

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