Product Details
| Alternative Name: | Apoptosis-associated speck-like protein containing a CARD, Caspase recruitment domain-containing protein 5, CARD-5, PYD and CARD domain-containing protein, PYCARD, Target of methylation-induced silencing, TMS1 |
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| Host: | Rabbit |
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| Immunogen: | Synthetic peptide corresponding to aa 182-195 of human ASC . |
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| UniProt ID: | Q9ULZ3 |
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| Species reactivity: | Human
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| Applications: | ICC, WB
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| Recommended Dilutions/Conditions: | Western Blot (1µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~25kDa by Western blot. |
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| Purity Detail: | Peptide affinity purified. |
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| Formulation: | Liquid. In PBS containing 0.02% sodium azide. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | ASC (Apoptosis-associated speck-like protein containing a CARD domain) is a bipartite protein comprising two protein-protein interaction domains, a Pyrin domain (PYD) and a caspase recruitment domain (CARD). Proteins containing these domains play pivotal roles in regulating apoptosis and immune response pathways. Reported functions of ASC include apoptosis, activation of inflammatory caspases and regulation of NFκB. |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of ASC in HL60 whole cell lysate in the absence (A) or presence (B) of blocking peptide (2287P) with ASC antibody at 1µg/ml.
Immunocytochemistry analysis of ASC in HL60 cells with ASC antibody at 5µg/ml.
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Product Literature References
Resolvin E1 attenuates doxorubicin-induced endothelial senescence by modulating NLRP3 inflammasome activation: L. Shamoon, et al.; Biochem. Pharmacol.
201, 115078 (2022),
Abstract;
AIM2 levels and DNA-triggered inflammasome response are increased in peripheral leukocytes of patients with abdominal aortic aneurysm: M. Wortmann, et al.; Inflamm. Res.
68, 337 (2019),
Abstract;
HSP70 is a negative regulator of NLRP3 inflammasome activation: P. Martine, et al.; Cell Death Dis.
10, 256 (2019),
Abstract;
Full Text
The IL-1/IL-1 receptor axis and tumor cell released inflammasome adaptor ASC are key regulators of TSLP secretion by cancer associated fibroblasts in pancreatic cancer: E. Brunneto, et al.; J. Immunother. Cancer
7, 45 (2019),
Application(s): IF and IHC in tumor cells ,
Abstract;
Full Text
Multitalented EspB of enteropathogenic Escherichia coli (EPEC) enters cells autonomously and induces programmed cell death in human monocytic THP-1 cells: D. Baumann, et al.; Int. J. Med. Microbiol.
308, 387 (2018),
Abstract;
NLRP3 Inflammasome-Related Proteins Are Upregulated in the Putamen of Patients With Multiple System Atrophy: F. Li, et al.; J. Neuropathol. Exp. Neurol.
77, 1055 (2018),
Abstract;
Nuclear Receptor Subfamily 1 Group D Member 1 Regulates Circadian Activity of NLRP3 Inflammasome to Reduce the Severity of Fulminant Hepatitis in Mice: B. Pourcet, et al.; Gastroenterology
154, 1449 (2018),
Abstract;
Carbon monoxide regulates glycolysis-dependent NLRP3 inflammasome activation in macrophages: D.W. Lee, et al.; Biochem. Biophys. Res. Commun.
493, 957 (2017),
Abstract;
Expression of TLR2, NOD1, and NOD2 and the NLRP3 Inflammasome in Renal Tubular Epithelial Cells of Male versus Female Mice: S.E. DeWolf, et al.; Nephron
137, 68 (2017),
Abstract;
Full Text
MARK4 regulates NLRP3 positioning and inflammasome activation through a microtubule-dependent mechanism: X. Li, et al.; Nat. Commun.
8, 15986 (2017),
Abstract;
Full Text
TNF regulates transcription of NLRP3 inflammasome components and inflammatory molecules in cryopyrinopathies: M.D. McGeough, et al.; J. Clin. Invest.
127, 4488 (2017),
Abstract;
Full Text
Caspase-1 mediated interleukin-18 activation in neutrophils promotes the activity of rheumatoid arthritis in a NLRP3 inflammasome independent manner: Z. Yang, et al.; Joint Bone Spine
83, 282 (2016),
Abstract;
Danggui Buxue Tang Attenuates Tubulointerstitial Fibrosis via Suppressing NLRP3 Inflammasome in a Rat Model of Unilateral Ureteral Obstruction: L. Wang, et al.; BioMed. Res. Int.
2016, Article ID 9368483 (2016),
Application(s): Western blot,
Abstract;
Full Text
Growth Hormone Receptor Deficiency Protects against Age-Related NLRP3 Inflammasome Activation and Immune Senescence: O. Spadaro, et al.; Cell Rep.
14, 1571 (2016),
Abstract;
Full Text
Lysosomal storage and impaired autophagy lead to inflammasome activation in Gaucher macrophages: E. Aflaki, et al.; Aging Cell
15, 77 (2016),
Application(s): Immunoblotting,
Abstract;
NLRP3 Inflammasome Expression and Activation in Human Atherosclerosis: P. Varghese, et al.; J. Am. Heart. Assoc.
5, e003031 (2016),
Application(s): Immunohistochemistry,
Abstract;
Full Text
NOX4-dependent fatty acid oxidation promotes NLRP3 inflammasome activation in macrophages: J.S. Moon, et al.; Nat. Med.
22, 1002 (2016),
Abstract;
Full Text
A small-molecule inhibitor of the NLRP3 inflammasome for the treatment of inflammatory diseases: R.C. Coll, et al.; Nat. Med.
21, 248 (2015),
Application(s): Western Blotting,
Abstract;
Anti-Inflammatory Effect of Emodin via Attenuation of NLRP3 Inflammasome Activation: J. W. Han, et al.; Int. J. Mol. Sci.
16, 8102 (2015),
Application(s): Western Blot,
Abstract;
Full Text
Estrogen suppresses hepatocellular carcinoma cells through ERβ-mediated upregulation of the NLRP3 inflammasome: Q. Wei, et al.; Lab. Invest.
95, 804 (2015),
Application(s): Immunohistochemistry, Western Blot,
Abstract;
Genipin suppresses NLRP3 inflammasome activation through uncoupling protein-2: V. Rajanbabu, et al.; Cell. Immunol.
297, 40 (2015),
Application(s): Western Blot,
Abstract;
Oligomerized CARD16 promotes caspase-1 assembly and IL-1β processing: T. Karasawa, et al.; FEBS Open Bio
5, 348 (2015),
Application(s): Immunocyotochemistry,
Abstract;
Role of NLRP3 Inflammasomes for Rhabdomyolysis-induced Acute Kidney Injury: T. Komada, et al.; Sci. Rep.
5, 10901 (2015),
Application(s): Western Blot,
Abstract;
Full Text
Staphylococcus aureus Leukocidin A/B (LukAB) Kills Human Monocytes via Host NLRP3 and ASC when Extracellular, but Not Intracellular: J.H. Melehani, et al.; PLoS Pathog.
11, e1004970 (2015),
Application(s): Western Blot,
Abstract;
Full Text
The ketone metabolite β-hydroxybutyrate blocks NLRP3 inflammasome-mediated inflammatory disease: Y.H. Youm, et al.; Nat. Med.
21, 263 (2015),
Application(s): Cell Culture, Western Blotting,
Abstract;
Multiple binding sites on the ASC pyrin domain allow self-association and interaction with NLRP3: P.R. Vajjhala, et al.; J. Biol. Chem.
287, 41732 (2012),
Application(s): Western-blotting using ASC-transfected HEK 293T cells,
Abstract;
Full Text
The Cytokine Release Inhibitory Drug CRID3 Targets ASC Oligomerisation in the NLRP3 and AIM2 Inflammasomes: R.C. Coll, et al.; PLoS One
6, e29539 (2011),
Abstract;
Varicella-zoster virus infection triggers formation of an interleukin-1β (IL-1β)-processing inflammasome complex: A.M. Nour, et al.; J. Biol. Chem.
286, 17921 (2011),
Application(s): Western-blotting using human lung embryonic fibroblasts and human melanoma cells,
Abstract;
Full Text
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