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AIF (IN) polyclonal antibody

ADI-905-168-100 100 µg 374.00 USD
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Product Specification

Alternative Name:Apoptosis-inducing factor
Immunogen:Synthetic peptide corresponding to a portion of human AIF. The sequence is conserved in mouse and rat AIF.
UniProt ID:O95831
Species reactivity:Human, Mouse, Rat
Applications:IHC, WB
Recommended Dilutions/Conditions:Immunohistochemistry (10µg/ml)
Western Blot (0.25-1.0µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~67kDa by Western blot.
Purity Detail:Ion exchange purified.
Formulation:Liquid. In PBS containing 0.02% sodium azide.
Shipping:Blue Ice Not Frozen
Long Term Storage:+4°C
Scientific Background:Apoptosis inducing factor (AIF) is a mitochondrial inter-membrane flavoprotein that is ubiquitously expressed both in normal tissues and in a variety of cancer cell lines. AIF is synthesized as a nonapoptogenic precursor in the cytoplasm and efficiently imported into the mitochondrial inter-membrane space. Mature AIF protein is normally confined to the mitochondrial inter-membrane space. In a variety of different apoptosis-inducing conditions AIF translocates through the outer mitochondrial membrane to the cytosol and to the nucleus. Extra-mitochondrial AIF induces nuclear chromatin condensation and large scale (~50kb) DNA fragmentation.
905-168 WB
Western blot analysis of AIF in K562 cell lysate (A), rat heart (B), and mouse heart (C) tissue lysates with AIF antibody (IN) at 1µg/ml.
905-168 IHC
Immunohistochemistry analysis of AIF in human retina with AIF antibody at 10µg/ml.
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905-168 WB 905-168 IHC

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Apoptosis-inducing factor
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Apoptosis-inducing factor Polyclonal antibody
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