5,6-dihydro PGI₂, pAb

Alternative Name:	Prostacyclin, PGI2, PGI

Formulation:	Liquid. Contains sodium azide.

Immunogen:	5,6-dihydro-Prostaglandin I2 conjugated to human albumin.

Source/Host:	From rabbit.

Specificity:	Species independent.

Application:	EIA Optimal conditions must be determined individually for each application.

Long Term Storage:	-20°C

Miscellaneous/General:	Prostacyclin (PGI2) is involved in platelet aggregation, vasoconstriction, and reproductive functions. PGI2 has a half life of 60 minutes in plasma but only 2 to 3 minutes in buffer. The production of PGI2 is typically monitored by measurement of 6-keto-PGF1alpha and 2,3-dinor-6-keto-PGF1alpha. 6-keto-PGF1alpha is produced by the non-enzymatic hydration of PGI2, and is further metabolized in urine to the 2,3-dinor derivative. A number of pharmaceuticals alter and/or inhibit the synthesis of PGI2. Methods to measure PGI2 in urine typically involve HPLC, gas chromatography/mass spectrometry, or radioimmunoassay and enzyme immunoassay for 6-keto-PGF1alpha. The conversion of PGI2 into 6-keto-PGF1alpha and 2,3-dinor-6-keto-PGF1alpha is shown below.