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TNF-α (human), ELISA kit

Highly sensitive ELISA for measuring TNF-α, the primary mediator of immune regulation.
 
ADI-901-099 5x96 wells 1,881.00 USD
Do you need bulk/larger quantities?
 
Alternative size available: ADI-900-099 (1x96 wells)
  • Highly sensitive ELISA, measuring as liitle as 8.4 pg/ml
  • High throughput format with results for up to 39 samples in duplicate in just 4 hours
  • Stable liquid color coded reagents and pre-coated plates reduce errors
  • Fully quantitative results that surpass semi-quantitative Western blot analysis
  • Save with bulk kit package!
The TNF-α (human), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 4 hours. Absorbance is read at 450 nm. Assay up to 39 samples in duplicate per 96-well plate. The broad dynamic range makes assaying different pathologies easy. The larger package size offers a lower cost-per-test.

Product Specification

Alternative Name:Tumor necrosis factor-α, TNFSF 2
 
Sensitivity:8.43 pg/ml (range 15.63 - 1,000 pg/ml)
 
Assay Time:4 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of human TNF-α in culture supernatants, plasma, and serum.
 
Species reactivity:Human
 
Use/Stability:Store all components at +4°C, except standard at -20°C.
 
Shipping:Shipped on Blue Ice
 
Kit/Set Contains:Microtiter plate, Conjugate, Antibody, Assay buffer 13 concentrate, Wash buffer concentrate, Standard, TMB Substrate, Stop solution 2
 
Scientific Background:Tumor Necrosis Factor-α (TNF-α) is a 17.5 kDa, 157 amino acid protein that is a potent lymphoid factor, which exerts cytotoxic effects on a wide range of tumor cells and other target cells. TNF-α has been suggested to play a pro-inflammatory role and has been detected in synovial fluid of patients with rheumatoid arthritis. It is the primary mediator of immune regulation. The biosynthesis of TNF-α is tightly controlled, being produced in extremely small quantities in quiescent cells, but is a major secreted factor in activated cells.
 
UniProt ID:P01375
 

Product Literature References

Activating transcription factor-3 induction is involved in the anti-inflammatory action of berberine in RAW264.7 murine macrophages: Y.A. Bae, et al.; Korean J. Physiol. Pharmacol. 20, 415 (2016), Application(s): ELISA (to find proinflammatory cytokine production), Abstract; Full Text
The Effect of Onion Peel Extract on Inflammatory Mediators in Korean Overweight and Obese Women: K.A. Kim & J.E. Yim; Clin. Nutr. Res. 5, 261 (2016), Application(s): Plasma samples, Abstract; Full Text
Vitamin D2 suppresses amyloid-β 25-35 induced microglial activation in BV2 cells by blocking the NF-κB inflammatory signaling pathway: S. Raha, et al.; Life Sci. 161, 37 (2016), Application(s): Assessment of the inhibitory effect of VD2 on the production of pro-inflammatory cytokines, Abstract;
Ginseng marc-derived low-molecular weight oligosaccharide inhibits the growth of skin melanoma cells via activation of RAW264.7 cells: J.Y. Seo, et al.; Int. Immunopharmacol. 29, 344 (2015), Application(s): Quantitation of cytokine levels in cultural supernates, Abstract;
Association between obesity-related adipokines and colorectal cancer: A case-control study and meta-analysis: R.K. Joshi, et al.; World J. Gastroenterol. 20, 7941 (2014), Application(s): Quantification of TNF-α in serum samples, Abstract; Full Text
Genetic damage in patients with chronic kidney disease, peritoneal dialysis and haemodialysis: a comparative study: A. Rangel-Lopez, et al.; Mutagenesis 28, 219 (2013), Application(s): ELISA using human serum, Abstract; Full Text
Effects of red orange juice intake on endothelial function and inflammatory markers in adult subjects with increased cardiovascular risk: S. Buscemi, et al.; Am. J. Clin. Nutr. 95, 1089 (2012), Application(s): Levels of IL-6 in human serum, Abstract; Full Text

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