Alternative size available: ADI-900-091 (1x96 wells)
Non-radioactive measurement of isoprostane in serum and plasma
Flexible options, either overnight or same day results
High sensitivity, measuring as little as 40 pg/ml
High throughput format with results for up to 39 samples in duplicate in less than 3 hours
Save with bulk kit package!
The measurement of F2-isoprostanes has emerged as one of the most reliable approaches to assess oxidative stress status in vivo, providing an important tool to explore the role of oxidative stress in the pathogenesis of human disease. The is a colorimetric competitive immunoassay kit for the quantitative determination of 8-iso-Prostaglandin F2α in biological fluids such as plasma, serum and tissue. To measure isoprostane in urine or culture supernatants, use the 8-iso-PGF2α EIA Kit.
8-iso-PGF2α is produced in vivo by both non-cyclooxygenase and cyclooxygenase dependant mechanisms from arachidonic acid. It has been shown to be a potent vasoconstrictor, a potential mediator of hepatorenal syndrome and a mutagen in 3T3 cells and vascular smooth muscle cells. 8-iso-PGF2α has been shown to circulate in plasma and is excreted in urine.
Product Specification
Alternative Name:
8-iso-Prostaglandin F2α
Sensitivity:
40.0 pg/ml (range 160 - 100,000 pg/ml)
Assay Time:
<3 hours or Overnight + 45 min
Applications:
ELISA, Colorimetric detection
Application Notes:
For the quantitative determination of 8-iso-PGF2α in plasma, serum, and tissue from any species. Cited sample types include cell lysate and urine.
Prostaglandin F2α (PGF2α) is formed in a variety of cells from PGH2, which itself is synthesized from arachidonic acid by the enzyme prostaglandin synthetase. PGF2α is often viewed as an antagonist to PGE2 due to their opposing effects on various tissues. PGF2α is a potent bronchoconstrictor and has been implicated in asthma attacks. PGF2α is also involved in reproductive functions including corpus luteum regulation, uterine contractions, and sperm motility. This has led to its use in terminating pregnancies and inducing labor at term. High levels of PGF2α have also been associated with preeclampsia.
Product Literature References
Placental antioxidant enzyme status and lipid peroxidation in pregnant women with type 1 diabetes: the effect of vitamin C and E supplementation : P.C. Johnston, et al.; J. Diabetes Complications 30, 109 (2016), Application(s): Measurement of placental 8-iso-Prostaglandin F2α , Abstract;
The activity of serum 8-iso-prostaglandin F2a as oxidative stress marker in patients with diabetes mellitus type 2 and associated dyslipidemic hyperglycemia: M.H. Mukhtar, et al.; J. Diab. Mellitus 6, 318 (2016), Application(s): ELISA using human serum samples, Full Text
A randomized clinical trial of ascorbic acid in open abdominal aortic aneurysm repair: M.J. Duffy, et al.; Intensive Care Med. Exp. 3, 20 (2015), Application(s): ELISA using human plasma, Abstract; Full Text
CYBA (p22phox) variants associate with blood pressure and oxidative stress markers in hypertension: a replication study in populations of diverse altitudes: R. Kumar, et al.; Hypertens. Res. 38, 498 (2015), Application(s): ELISA using human plasma, Abstract;
Mitochondrial translocation of human telomerase reverse transcriptase in cord blood mononuclear cells of newborns with gestational diabetes mellitus mothers: P. Li, et al.; Diabetes Res. Clin. Pract. 103, 310 (2014), Application(s): ELISA using human plasma, Abstract;
Comparison of serum F2 isoprostane levels in diabetic patients and diabetic patients infected with Burkholderia pseudomallei: S. Puthucheary, et al. ; Singapore Med. J. 49, 117 (2008), Application(s): EIA using human serum, Abstract;
Lipid peroxidation products do not activate hepatic stellate cells: W. Lin, et al. ; Toxicology 253, 36 (2008), Application(s): EIA using rat tissue, Abstract;
Cigarette smokers have decreased lymphocyte and platelet alpha-tocopherol levels and increased excretion of the gamma-tocopherol metabolite gamma-carboxyethyl-hydroxychroman (gamma-CEHC): Y.M. Jeanes, et al. ; Free Radic. Res. 38, 861 (2004), Application(s): EIA using human plasma, Abstract;
Differential oxidant potential of carcinogenic and weakly carcinogenic estrogens: Involvement of metabolic activation and cytochrome P450: M.M. Patel, et al. ; J. Biochem. Mol. Toxicol. 18, 37 (2004), Application(s): EIA using human & hamster cell lysates, Abstract;
Critical role of oxidative stress in estrogen-induced carcinogenesis: H.K. Bhat, et al. ; PNAS 100, 3913 (2003), Application(s): EIA using hamster cell lysates, Abstract;
Treadmill exercise in mice increases intestinal lymphocyte loss via apoptosis: L. Hoffman-Goetz, et al. ; Acta Physiol. Scand. 179, 289 (2003), Application(s): EIA using mouse plasma, Abstract;
Effects of vitamin C and vitamin E on in vivo lipid peroxidation: results of a randomized controlled trial: H.Y. Huang, et al. ; Am. J. Clin. Nutr. 76, 549 (2002), Application(s): EIA using human urine, Abstract;
Oxidant stress in sled dogs subjected to repetitive endurance exercise: K.W. Hinchcliff, et al. ; Am. J. Vet. Res. 61, 512 (2000), Application(s): EIA using canine plasma, Abstract;
