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Cortisol ELISA kit

Widely used sensitive cortisol ELISA kit with a large variety of sample types and highly reproducible results for stress, autoimmune disease and cancer research.
 
ADI-901-071 5x96 wells 1,314.00 USD
Do you need bulk/larger quantities?
 
Alternative size available: ADI-900-071 (1x96 wells)
  • Senstitive measurement of cortisol, detecting as little as 56.72 pg/ml
  • Highly cited in peer reviewed literature
  • Reproducible results day-after-day and lot-after-lot
  • Fully quantitative results that surpass semi-quantitative Western blot analysis
  • Easy-to-use pre-coated plates and liquid color-coded reagents save time and reduce errors
  • Save with bulk kit package!
The Cortisol EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 3 hours. Absorbance is read at 405 nm. This kit is highly cited in peer-reviewed publications. The ready-to-use liquid color-coded reagents provided are safe, non-radioactive, and reduce error in the lab. The broad dynamic range lets you accurately measure Cortisol levels in a variety of sample matrices.
ADI-901-071 std curve
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ADI-901-071 std curve

Product Specification

Sensitivity:56.72 pg/ml (range 156 - 10,000 pg/ml)
 
Assay Time:3 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of Cortisol in culture supernatants, plasma, serum, saliva, and urine from any species. Cited sample type includes feces.
 
Species reactivity:Species independent
 
Crossreactivity:Cortisol (100%), Prednisolone (122.35%), Corticosterone (27.68%), 11-deoxycortisol (4.0%), Progesterone (3.64%), Prednisone (0.85%), Testosterone (0.12%) and <0.10%: Androstenedione, Cortisone, Estradiol
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Kit/Set Contains:GxM Microtiter plate, Conjugate, Antibody, Assay buffer concentrate, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution, Steroid displacement reagent
 
Scientific Background:Cortisol (hydrocortisone, compound F) is a steroid hormone synthesized from cholesterol. It is the primary glucocorticoid produced and secreted by the adrenal cortex. Cortisol is found in the blood either as free cortisol, or bound to corticosteroid-binding globulin (CBG). Serum levels are highest in the early morning and decrease throughout the day. Cortisol is known to regulate metabolism, promoting gluconeogenesis, liver glycogen deposition, and the reduction of glucose utilization. Cortisol production is positively correlated with stress, resulting in increased blood pressure, and exerts immunosuppressive effects on the immune system.
 
Technical Info/Product Notes:For an overview of cited samples, please click here
 

Product Literature References

Glucose and insulin modulate sickness responses in male Siberian hamsters: E.D. Carlton & G.E. Demas; Gen. Comp. Endocrinol. 242, 83 (2017), Application(s): Cortisol levels measured in hamster serum, Abstract;
Peripheral regulation of stress and fear responses in pigs from tail-biting pens: M. Zupan, et al.; R. Bras. Zootec. 46, 33 (2017), Application(s): Measurement of cortisol in pig saliva samples, Full Text
Puerarin ameliorated the behavioral deficits induced by chronic stress in rats: Z.K. Qiu, et al.; Sci. Rep. 7, 6266 (2017), Application(s): EIA on rat tissue homogenate, Abstract; Full Text
Relocation stress induces short-term fecal cortisol increase in Tonkean macaques (Macaca tonkeana): C. Cinque, et al.; Primates 58, 315 (2017), Application(s): Measurement of fecal cortisol metabolite concentrations in Tonkean macaques, Abstract;
Stress and immunological response of heifers divergently ranked for residual feed intake following an adrenocorticotropic hormone challenge: A.K. Kelly, et al.; J. Anim. Sci. Biotechnol. 8, 65 (2017), Application(s): Beef heifer plasma samples, Abstract; Full Text
Bifidobacterium longum 1714 as a translational psychobiotic: modulation of stress, electrophysiology and neurocognition in healthy volunteers: A.P. Allen, et al.; Transl. Psychiatry 6, e939 (2016), Application(s): Salivary samples, Abstract;
Effects of turmeric curcuminoids and metformin against central sensitivity to pain in mice: S. Verma, et al.; J. Tradit. Complement. Med. 7, 145 (2016), Application(s): Mouse tail plasma cortisol levels were measured, Abstract; Full Text
Efficacy of oral meloxicam suspension for prevention of pain and inflammation following band and surgical castration in calves: M. Olson, et al.; BMC Vet. Res. 12, 102 (2016), Application(s): ELISA using blood samples, Abstract; Full Text
Expansion of bone marrow adipose tissue during caloric restriction is associated with increased circulating glucocorticoids and not with hypoleptinemia: W.P. Cawthorn, et al.; Endocrinology 157, 508 (2016), Application(s): Determination of cortisol levels in blood and serum, Abstract; Full Text
Expression and regulation of 11β-hydroxysteroid dehydrogenase type 1 in first trimester human decidua cells: Implication in preeclampsia: L. Funghi, et al.; Mol. Cell. Endocrinol. 437, 163 (2016), Application(s): Enzymatic activity, cell homogenates, Abstract;
Intermittent Suckling Causes a Transient Increase in Cortisol That Does Not Appear to Compromise Selected Measures of Piglet Welfare and Stress: D.L. Turpin, et al.; Animals (Basel) 6, e24 (2016), Application(s): Measured plasma cortisol levels, Abstract; Full Text
Intermittent Suckling in Combination with an Older Weaning Age Improves Growth, Feed Intake and Aspects of Gastrointestinal Tract Carbohydrate Absorption in Pigs after Weaning: D.L. Turpin, et al.; Animals (Basel) 6, e66 (2016), Application(s): Analysis of cortisol in piglet plasma, Abstract; Full Text
Oxysterol-related-binding-protein related Protein-2 (ORP2) regulates cortisol biosynthesis and cholesterol homeostasis: T. Escajadillo, et al.; Mol. Cell. Endrocrinol. 427, 73 (2016), Application(s): Measured cortisol levels, Abstract;
Physiological and psychological responses in Fire Instructors to heat exposures: P.W. Watt, et al.; J. Therm. Biol. 58, 106 (2016), Application(s): Cortisol levels were measured in plasma samples, Abstract;
Psychological Profiles in the Prediction of Leukocyte Telomere Length in Healthy Individuals: L. Starnino, et al.; PLoS One 11, e0165482 (2016), Application(s): Measuring human salivary cortisol levels, Abstract; Full Text
Stress in the neighborhood: Tissue glucocorticoids relative to stream quality for five species of fish: G.D. King, et al.; Sci. Total Environ. 547, 87 (2016), Application(s): Cortisol levels measured in fish tissue, Abstract;
The immune responses and expression of metallothionein (MT) gene and heat shock protein 70 (HSP 70) in juvenile rockfish, Sebastes schlegelii, exposed to waterborne arsenic (As3+): J.H. Kim, et al.; Environ. Toxicol. Pharmacol. 47, 136 (2016), Application(s): Measurement of plasma cortisol concentration, Abstract;
The immune responses in juvenile rockfish, Sebastes schlegelii for the stress by the exposure to the dietary lead (II): J.H. Kim, et al.; Environ. Toxicol. Pharmacol. 46, 211 (2016), Application(s): Plasma cortisol concentration measurement, Abstract;
The toxic effects on the stress and immune responses in juvenile rockfish, Sebastes schlegelii exposed to hexavalent chromium: J.H. Kim, et al.; Environ. Toxicol. Pharmacol. 43, 128 (2016), Application(s): Measurement of plasma cortisol concentration in rockfish, Abstract;
Using physiological and zootechnical profiles to evaluate welfare in farmed rainbow trout Oncorhynchus mykiss (Walbaum) under stressful conditions: C. Delfosse, et al.; Aquacult. Int. 24, 1449 (2016), Application(s): Cortisol levels in trout plasma, Abstract;
A potent and selective 11β-hydroxysteroid dehydrogenase type 1 inhibitor, SKI2852, ameliorates metabolic syndrome in diabetic mice models: H. Oh, et al.; Eur. J. Pharmacol. (2015), Application(s): Ex vivo analysis in mice tissue, Abstract;
Decreased Cortisol and Pain in Breast Cancer: Biofield Therapy Potential: A. Running; Evid. Based Complement. Alternat. Med. 2015, 870640 (2015), Application(s): ELISA using mouse feces, Abstract; Full Text
Effect of floor type on the performance, physiological and behavioural responses of finishing beef steers: B. Earley, et al.; Acta. Vet. Scand. 57, 73 (2015), Application(s): Quantitative determination of cortisol in plasma, Abstract; Full Text
Effects of Oxytocin Administration on the Response of Piglets to Weaning: J. L. Rault, et al.; Animals 5, 545 (2015), Application(s): ELISA using pig plasma, Full Text
Identification of a novel 11β-HSD1 inhibitor from a high-throughput screen of natural product extracts: S.B. Park, et al.; Pharmacol. Res. 102, 245 (2015), Application(s): Ex vivo assay for 11β-HSD1 activity, Abstract;
Mares prefer the voices of highly fertile stallions: A. Lemasson, et al.; PLoS One 10, e0118468 (2015), Application(s): ELISA using equine plasma, Abstract; Full Text
A test of maternal programming of offspring stress response to predation risk in threespine sticklebacks: B.C. Mommer, et al.; Physiol. Behav. 122, 222 (2013), Application(s): EIA using fish plasma, Abstract;
Sphingosine-1-phosphate rapidly increases cortisol biosynthesis and the expression of genes involved in cholesterol uptake and transport in H295R adrenocortical cells: N.C. Lucki, et al.; Mol. Cell Endocrinol. 348, 165 (2012), Application(s): EIA using cell culture supernatants, Abstract; Full Text
Unravelling complex associations between testosterone and parasite infection in the wild: V.O. Ezenwa, et al.; Functional Ecology 26, 123 (2012), Application(s): ELISA using gazelle feces, Abstract;
Comparison of evaporation techniques for the preparation of salivary cortisol for analysis by liquid chromatography-electrospray tandem mass spectrometry: E. Nelson, et al. ; Clin. Biochem. 41, 1413 (2008), Application(s): EIA using human saliva, Abstract;
Expression of arginine vasotocin in distinct preoptic regions is associated with dominant and subordinate behaviour in an African cichlid fish: R. Fernald, et al. ; Proc. Biol. Sci. 275, 2393 (2008), Application(s): EIA using fish plasma, Abstract;
Stress assessment in acute care department nurses by measuring interleukin-8: H. Fukuda, et al. ; Int. Nurs. Rev. 55, 407 (2008), Application(s): EIA using human urine, Abstract;
Attenuation of the glucocorticoid response during Ad5IL-12 adenovirus vector treatment enhances natural killer cell-mediated killing of MHCclass I-negative LNCaP prostate tumors: T. Braciak, et al. ; Cancer Res. 67, 2290 (2007), Application(s): EIA using mouse serum, Abstract;
Adrenal hormones mediate melatonin-induced increases in aggression in male Siberian hamsters (Phodopus sungorus): G.E. Demas, et al.; Horm. Behav. 46, 582 (2004), Application(s): Blood serum testosterone and serum cortisol from Siberian hamsters, Abstract;
Behavioural and neuroendocrine adaptations to repeated stress during puberty in male golden hamsters: J.C. Wommack, et al. ; J. Neuroendocrinol. 16, 767 (2004), Application(s): EIA using hamster plasma, Abstract;
Repeated social stress and the development of agonistic behavior: individual differences in coping responses in male golden hamsters: J.C. Wommack, et al. ; Physiol. Behav. 80, 303 (2003), Application(s): EIA using hamster serum, Abstract;

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