Direct cAMP ELISA kit

Most sensitive (0.037 pmol/ml) colorimetric kit for intracellular cAMP quantification.



ADI-901-066	5x96 wells	1'410.00 USD

Replaces Prod. #: BML-AK215

Trusted – regularly cited in peer-reviewed publications
Sensitive – optional acetylation protocol increases sensitivity 10-fold (0.037 pmol/ml)
Rapid – results in just 3 hours from up to 39 samples in duplicate
Easy-to-use – pre-coated plate and liquid color-coded reagents reduce errors
Simple – efficient and well-established sample handling protocols
Reliable – consistent lot-to-lot performance
Save with bulk kit package!

This is a colorimetric competitive immunoassay kit for the quantitative determination of intracellular cAMP in cells or tissues lysed in 0.1 M HCl, which is used to stop endogenous phosphodiesterase activity and stabilize the released cyclic AMP. The 0.1M HCl treated samples are then analyzed directly in a microtiter plate without extraction, drying and reconstitution. Sensitivity is increased 10-fold by acetylation (reagents included). Absorbance is read at 405 nm. The large signal-to-background ratio offers superior sensitivity compared to competitors.

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Product Specification

Alternative Name:	cyclic AMP

Sensitivity:	(non-acetylated) 0.39 pmol/ml; (acetylated) 0.037 pmol/ml (range (non-acetylated) 0.78 - 200 pmol/ml;(acetylated) 0.078 - 20 pmol/ml)

Kit/Set Contains:	GxR Microtiter plate, Conjugate, Antibody, 0.1M HCl, Neutralizing reagent, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution, Acetic anhydride, Triethylamine

Application:	For the quantitative determination of cAMP in cell lysates and tissue from any species. Cited sample types include cell cuture supernatant (ref. 1) and tissue culture (ref. 2).

Use/Stability:	Store all components at +4°, except standard and conjugate at -20°.

Miscellaneous/General:	Cyclic AMP (cAMP) is one of the most important “second messengers” involved as a modulator of physiological processes. A number of hormones are known to activate cAMP through the action of the enzyme Adenylate cyclase which converts ATP to cAMP. cAMP has been shown to be involved in the cardiovascular and nervous systems, immune mechanisms, cell growth and differentiation, and general metabolism.

Product Literature References

cAMP pulsing of denuded mouse oocytes increases meiotic resumption via activation of AMP-activated protein kinase.: Chen J., et al.; Reproduction 138, 759 (2009), Abstract;

Heat-Labile Enterotoxin Promotes Escherichia coli Adherence to Intestinal Epithelial Cells: P. Hardwidge, et al. ; J. Bacteriol. 191, 178 (2009), Application(s): EIA using porcine cell lysates, Abstract;

Androgens transduce the G-alpha s-mediated activation of protein kinase A in prostate cells: Y. Daaka, et al. ; Cancer Res. 68, 3225 (2008), Application(s): EIA using human cell lysates, Abstract;

beta-Arrestin-1 mediates glucagon-like peptide-1 signaling to insulin secretion in cultured pancreatic beta cells: J. Olefsky, et al. ; PNAS 105, 6614 (2008), Application(s): EIA using rat cell lysates, Abstract;

Cyclic Adenosine Monophosphate Regulation of Ion Transport in PorcineVocal Fold Mucosae: M. Sivasankar, et al.; Laryngoscope 118, 1511 (2008), (ref. 2), Abstract;

FMRP acts as a key messenger for dopamine modulation in the forebrain: M. Zhao, et al. ; Neuron 59, 634 (2008), Application(s): EIA using mouse cell lysates, Abstract;

Functional evidence for presence of lipid rafts in erythrocyte membranes: Gs-alpha in rafts is essential for signal transduction: Y. Takakuwa, et al. ; Am. J. Hematol. 83, 371 (2008), Application(s): EIA using human cell lysates, Abstract;

Regulation of G proteins by human 5-HT1a receptor TM3/i2 and TM5/i3 loop peptides: H.V. Thiagaraj, et al. ; Neurochem. Int. 50, 109 (2007), Application(s): EIA using human cell lysates, Abstract;

Co-occurrence of Two Partially Inactivating Polymorphisms of MC3R Is Associated With Pediatric-Onset Obesity: N. Feng, et al. ; Diabetes 54, 2663 (2005), Application(s): EIA using human cell lysates, Abstract;

The hrpK Operon of Pseudomonas syringae pv. tomato DC3000 Encodes Two Proteins Secreted by the Type III (Hrp) Protein Secretion System: HopB1 and HrpK, a Putative Type III Translocator: T. Petnicki-Ocwieja, et al. ; J. Bacteriol. 187, 649 (2005), Application(s): EIA using plant cell lysates, Abstract;

High-salt diet impairs vascular relaxation mechanisms in rat middle cerebral arteries: J.H. Lombard, et al. ; Am. J. Physiol. Heart Circ. Physiol. 284, H1124 (2003), Application(s): EIA using rat cell lysates, Abstract;

In Utero and Lactational Exposure to 2,3,7,8-Tetrachlorodibenzo-p-dioxin in the C57BL/6J Mouse Prostate: Lobe-Specific Effects on Branching Morphogenesis: K. Ko, et al.; Toxicol. Sci. 70, 227 (2002), Abstract;

Lysine 270 in the Third Intracellular Domain of the Oxytocin Receptor is an Important Determinant for G{alpha}q Coupling Specificity: M. Yang, et al. ; Mol. Endocrinol. 16, 814 (2002), Application(s): EIA using monkey cell lysates, Abstract;

Effect of Tumor Necrosis Factor-{{alpha}}, Interferon-{{gamma}}, and Transforming Growth Factor-{beta} on Adipogenesis and Expression of Thyrotropin Receptor in Human Orbital Preadipocyte Fibroblasts: R.W. Valyasevi, et al.; J. Clin. Endocrinol. Metab. 86, 903 (2001), (ref. 1), Abstract;