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Testosterone ELISA kit

ADI-901-065 5x96 wells 1,313.00 USD
Do you need bulk/larger quantities?
Alternative size available: ADI-900-065 (1x96 wells)
  • Sensitive measurement of testosterone, detecting as little as 5.67 pg/ml
  • Simple and ready-to-use liquid color-coded reagents reduces error
  • Steroid displacement reagent provided to assay free or total testosterone
  • High throughput format with results in <3 hours
  • Excellent consistency lot-after-lot ideal for longitudinal studies
  • Fully quantitative results that surpass semi-quantitative Western blot analysis
  • Save with bulk kit package!
The Testosterone EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 3 hours. Absorbance is read at 405 nm. Ready-to-use liquid color-coded reagents are provided to reduce error. The reproducible results lot-after-lot provide excellent consistency, ideal for longitudinal studies.

Product Specification

Sensitivity:5.67 pg/ml (range 7.81 - 2,000 pg/ml)
Assay Time:3 hours
Applications:ELISA, Colorimetric detection
Application Notes:For the quantitative determination of testosterone in culture supernatants, plasma, serum, and saliva from any species. Not suitable for human serum and plasma. For those samples use product ADI-901-176. Cited sample type includes tissue and feces.
Species reactivity:Species independent
Crossreactivity:Testosterone (100%), 19-hydroxytestosterone (14.6%), Androstendione (7.20%), Dehydroepiandrosterone (0.72%), Estradiol (0.40%) and <0.001%: Dihydrotestosterone, Estriol, Aldosterone, Corticosterone, Cortisol, Cortisone, Estrone, Progesterone, Pregenolone
Shipping:Blue Ice Not Frozen
Long Term Storage:+4°C
Contents:GxM Microtiter plate, Conjugate, Antibody, Assay buffer 3, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution, Steroid displacement reagent
Scientific Background:Testosterone is one of the most important androgens secreted into the bloodstream and is synthesized from pregnenolone which is itself formed from cholesterol. Testosterone is the main androgen secreted by the Leydig cells of the testes and effects both primary and secondary sexual development such as muscle mass and sex drive. In adult humans, testosterone circulates in plasma predominately bound to proteins, including specific sex hormone binding globulin (SHBG) and nonspecific proteins such as albumin.
Technical Info/Product Notes:For an overview of cited samples, please click here

Application Note:
Validation of Two Steroid Hormone ELISA Kits with Ovine Blood Samples.

Product Literature References

Environmental warming and feminization of one of the largest sea turtle populations in the world: M.P. Jensen, et al.; Curr. Biol. 28, 154 (2018), Application(s): Testoterone levels in sea turtle plasma, Abstract;
Experimental manipulation of a signal trait reveals complex phenotype-behaviour coordination: I. Levin, et al.; Sci. Rep. 8, 15533 (2018), Abstract; Full Text
Inflammatory Responses are Sex Specific in Chronic Hypoxic-Ischemic Encephalopathy: A. Al Mamun, et al.; Cell Transplant. 27, 1328 (2018), Application(s): EIA using human serum, Abstract; Full Text
Multiple aspects of male germ cell development and interactions with Sertoli cells require inositol hexakisphosphate kinase-1: C. Fu, et al.; Sci. Rep. 8, 7039 (2018), Abstract; Full Text
Songbird chemical signals reflect uropygial gland androgen sensitivity and predict aggression: implications for the role of the periphery in chemosignaling: D.J. Whittaker, et al.; J. Comp. Physiol. A Neuroethol. Sens. Neural. Behav. Physiol. 204, 5 (2018), Abstract;
Consistent individual variation in day, night, and GnRH-induced testosterone concentrations in house sparrows (Passer domesticus): K.B. Needham, et al.; Gen. Comp. Endocrinol. 246, 211 (2017), Application(s): Testosterone levels in house sparrow plasma, Abstract;
Estrogen activation of microglia underlies the sexually dimorphic differences in Nf1 optic glioma-induced retinal pathology: J.A. Toonen, et al.; J. Exp. Med. 214, 17 (2017), Abstract;
In vivo evaluation of hot water extract of Acorus gramineus root against benign prostatic hyperplasia: J.M. Moon, et al.; BMC Infect. Dis. 17, 414 (2017), Abstract; Full Text
Loss of predator aversion in female rats after Toxoplasma gondii infection is not dependent on ovarian steroids: S. Abdulai-Saiku & A. Vyas; Brain Behav. Immun. 65, 95 (2017), Application(s): ELISA using male rat samples with T. Gondi infection , Abstract;
A potential mate influences reproductive development in female, but not male, pine siskins: H.E. Watts, et al.; Horm. Behav. 80, 39 (2016), Application(s): Measured testosterone levels in plasma of pine siskins, Abstract;
Aromatized testosterone attenuates contextual generalization of fear in male rats: J.F. Lynch 3rd, et al.; Horm. Behav. 84, 127 (2016), Application(s): Serum testosterone assays, Abstract;
Choosing a healthy mate: sexually attractive traits as reliable indicators of current disease status in house mice: P.C. Lopes, et al.; Anim. Behav. 111, 119 (2016), Application(s): Testosterone levels tested in mice,
Contrasting effects of opposite- versus same-sex housing on hormones, behavior and neurogenesis in a eusocial mammal: D.E. Peragine, et al.; Horm. Behav. 81, 28 (2016), Application(s): Measurement of rodent serum testosterone concentration, Abstract;
Corticosterone may interact with peripubertal development to shape adult resistance to social defeat: M.S. Latsko, et al.; Horm. Behav. 82, 38 (2016), Application(s): Serum testosterone levels were measured, Abstract;
Di-(2-ethylhexyl) phthalate inhibits testosterone level through disturbed hypothalamic-pituitary-testis axis and ERK-mediated 5α-Reductase 2: M. Ha, et al.; Sci. Total Environ. 563, 566 (2016), Application(s): Serum testosterone levels were measured, Abstract;
Differential effects of bisphenol A and estradiol on rat spermatogenesis’ establishment: V. Brouard, et al.; Reprod. Toxicol. 63, 49 (2016), Application(s): Measured plasmatic testosterone concentration, Abstract;
Effect of Sleep Deprivation on the Male Reproductive System in Rats: J.H. Choi, et al.; J. Korean Med. Sci. 31, 1624 (2016), Application(s): Rat blood sampling for testosterone measurements, Abstract; Full Text
Effects of aging on stress-related responses of serotonergic neurons in the dorsal raphe nucleus of male rats: N. Yamaguchi, et al.; Neurobiol. Stress 3, 43 (2016), Application(s): Measurement of testosterone in rat plasma, Abstract; Full Text
Endocrine, metabolic, and behavioral effects of and recovery from acute stress in a free-ranging bird: P. Deviche, et al.; Gen. Comp. Endocrinol. 6480, 30186 (2016), Application(s): Plasma ELISA, Abstract;
Environmental chemicals impact dog semen quality in vitro and may be associated with a temporal decline in sperm motility and increased cryptorchidism: R.G. Lea, et al.; Sci. Rep. 6, 31281 (2016), Application(s): Testosterone analysis, dog testis explants, Abstract; Full Text
Estrogenic modulation of fear generalization: J.F. Lynch 3rd, et al.; (2016), (PhD thesis), Application(s): Serum testosterone assays, trunk blood, Full Text
Heightened aggressive behavior in mice deficient in aldo-keto reductase 1a (Akr1a): T. Homma, et al.; Behav. Brain Res. 22, 319 (2016), Application(s): Testosterone levels in plasma were measured, Abstract;
Hypothalamic-pituitary-adrenal axis activity is not elevated in a songbird (Junco hyemalis) preparing for migration: C.M. Bauer, et al.; Gen. Comp. Endocrinol. 232, 60 (2016), Application(s): Testosterone levels measured in songbirds, Abstract;
Maternal testosterone and reproductive outcome in a rat model of obesity: L. Arnon, et al.; Theriogenology 86, 1042 (2016), Application(s): Measured rat hair testosterone levels, Abstract;
Phenotypic correlates of melanization in two Sceloporus occidentalis (Phrynosomatidae) populations: Behavior, androgens, stress reactivity, and ectoparasites: R.J. Seddon, et al.; Physiol. Behav. 163, 70 (2016), Application(s): Testosterone levels were measured in lizard plasma, Abstract;
Testosterone production by a leydig tumor cell line is suppressed by hyperthermia-induced endoplasmic reticulum stress in mice: J.H. Kim, et al.; Life Sci. 146, 184 (2016), Application(s): Measurement of testosterone in mouse mLTC-1 cell culture media, Abstract;
The endocrine disrupter effect of atrazine and glyphosate on Biomphalaria alexandrina snails: N. Omran & W. Salama; Toxicol. Ind. Health 32, 656 (2016), Application(s): Analysis of endocrine disruptors in snails, Abstract;
Why do migratory birds sing on their tropical wintering grounds?: M.C. Sorensen, et al.; Am. Nat. 187, E65 (2016), Abstract;
A CYP19 Based Sex Determination and Monosex Production in Aquaculture Species Oreochromis niloticus L. and a Cyprinid Cyprinus carpio L.: A.K. Singh, et al.; Fish. Aquac. J. 6, 1 (2015), Application(s): ELISA using fish serum, Full Text
Advanced seasonal reproductive development in a male urban bird is reflected in earlier plasma luteinizing hormone rise but not energetic status: S. Davies, et al.; Gen. Comp. Endocrinol. 224, 1 (2015), Application(s): ELISA using bird plasma, Abstract;
Epigallocatechin gallate exacerbates fluoride-induced oxidative stress mediated testicular toxicity in rats through the activation of Nrf2 signaling pathway: S. Thangapandiyan; Asian Pac. J. Reprod. 4, 272 (2015), Application(s): ELISA using rat plasma,
First Assessment of the Sex Ratio for an East Pacific Green Sea Turtle Foraging Aggregation: Validation and Application of a Testosterone ELISA: C.D. Allen, et al.; PLoS One 10, e0138861 (2015), Application(s): Used to determine testosterone concentration in each extracted plasma sample, Abstract; Full Text
Food, stress, and circulating testosterone: Cue integration by the testes, not the brain, in male zebra finches (Taeniopygia guttata): S. E. Lynn, et al.; Gen. Comp. Endocrinol. 215, 1 (2015), Application(s): ELISA using zebra finch plasma , Abstract;
Mares prefer the voices of highly fertile stallions: A. Lemasson, et al.; PLoS One 10, e0118468 (2015), Application(s): ELISA using equine plasma, Abstract; Full Text
Negative energy balance in a male songbird, the Abert's Towhee, constrains the testicular endocrine response to luteinizing hormone stimulation: S. Davies, et al.; J. Exp. Biol. 218, 2685 (2015), Application(s): ELISA using bird plasma, Abstract; Full Text
Prenatal Testosterone Exposure Influences Neuronal Sensitivity to Pheromones in Female Mice: R.N. Thompson, et al; Biochem. Physiol. 4, 2168 (2015), Application(s): ELISA assay for testosterone in female mice, Full Text
Removal of reproductive suppression reveals latent sex differences in brain steroid hormone receptors in naked mole-rats, Heterocephalus glaber: A. Swift-Gallant, et al.; Biol. Sex Differ. 6, 31 (2015), Application(s): Measured Total T from serum, Abstract; Full Text
Actions of oestradiol and progesterone on the prostate in female gerbils: reversal of the histological effects of castration: M. Zanatelli, et al.; Reprod. Fertil. Dev. 26, 540 (2014), Abstract;
Alterations in the estrogen environment of the testis contribute to declining sperm production in aging rats: M. Clarke & C. Pearl; Syst. Biol. Reprod. Med. 60, 89 (2014), Application(s): Analysis of sperm production and hormone levels in rat testes by ELISA, Abstract;
Gender differences in adenine-induced chronic kidney disease and cardiovascular complications in rats: V. Diwan, et al.; Am. J. Physiol. Renal Physiol. 307, F1169 (2014), Application(s): Kidney disease analysis in rat plasma by ELISA, Abstract;
In vivo detection of fluctuating brain steroid levels in zebra finches: M. Ikeda, et al.; Cold Spring Harb. Protoc. 2014, 1267 (2014), Application(s): In vivo analysis of hormones in zebra finch brain, Abstract; Full Text
Regulation of plasma testosterone, corticosterone, and metabolites in response to stress, reproductive stage, and social challenges in a desert male songbird: P. Deviche, et al.; Gen. Comp. Endocrinol. 203, 120 (2014), Application(s): Quantified plasma testosterone, Abstract;
Chronic social isolation is associated with metabolic gene expression changes specific to mammary adipose tissue: P.A. Volden, et al.; Cancer Prev. Res. (Phila) 6, 634 (2013), Application(s): Measurement of corticosterone levels in tail blood of mice, Abstract; Full Text
Female preference for males depends on reproductive physiology in the African cichlid fish Astatotilapia burtoni: M.R. Kidd, et al.; Gen. Comp. Endocrinol. 180, 56 (2013), Application(s): ELISA using aquarium water, Abstract;
Gender-dimorphic regulation of skeletal muscle proteins in streptozotocin-induced diabetic rats: M. Choi, et al.; Cell Physiol. Biochem. 31, 408 (2013), Application(s): EIA using rat plasma, Abstract; Full Text
hCG-induced endoplasmic reticulum stress triggers apoptosis and reduces steroidogenic enzyme expression through activating transcription factor 6 in Leydig cells of the testis: S.J. Park, et al.; J. Mol. Endocrinol. 50, 151 (2013), Application(s): ELISA using culture supernatants, Abstract;
Perinatal testosterone exposure and maternal care effects on the female rat's development and sexual behaviour: A.P. Borrow, et al.; J. Neuroendocrinol. 25, 528 (2013), Application(s): EIA using rat plasma, Abstract;
Sex steroid hormones modulate responses to social challenge and opportunity in males of the monogamous convict cichlid, Amatitliana nigrofasciata: A. Sessa, et al.; Gen. Comp. Endocrinol. 189, 59 (2013), Application(s): EIA using fish plasma, Abstract;
The screening of everyday life chemicals in validated assays targeting the pituitary-gonadal axis: H. Tinwell, et al.; Regul. Toxicol. Pharmacol. 66, 184 (2013), Application(s): EIA using rat plasma, Abstract;
Effects of velvet antler with blood on bone in ovariectomized rats: S.H. Tseng, et al.; Molecules 17, 10574 (2012), Application(s): ELISA using ether extract of velvet antler, Abstract; Full Text
Isotocin regulates paternal care in a monogamous cichlid fish: L. O'Connell, et al.; Horm. Behav. 61, 725 (2012), Application(s): EIA using fish plasma , Abstract;
Non-photoperiodic regulation of reproductive physiology in the flexibly breeding pine siskin (Spinus pinus): H.E. Watts & T.P. Hahn; Gen. Comp. Endocrinol. 178, 259 (2012), Application(s): Measurement of testosterone levels in pine siskin plasma samples, Abstract; Full Text
Unravelling complex associations between testosterone and parasite infection in the wild: V.O. Ezenwa, et al.; Functional Ecology 26, 123 (2012), Application(s): ELISA using gazelle feces, Abstract;
Effects of suppressing gonadal hormones on response to novel objects in adolescent rats: D.L. Cyrenne, et al.; Horm. Behav. 60, 625 (2011), Application(s): ELISA using rat serum, Abstract; Full Text
Non-breeding gonadal testosterone production of male and female northern cardinals (Cardinalis cardinalis) following GnRH challenge: M.S. DeVries, et al.; Gen. Comp. Endocrinol. 174, 370 (2011), Application(s): Plasma testosterone in cardinals, Abstract;
Fecal Hormones Measured within Giant Pacific Octopuses Enteroctopus dofleini: S. Larson & R. Anderson; J. Aquat. Anim. Health 22, 152 (2010), Application(s): EIA using octopus feces, Abstract;
Measuring multiple hormones from a single water sample using enzyme immunoassays: C. Kidd, et al.; Gen. Comp. Endocrinol. 165, 277 (2010), Application(s): EIA using fish plasma and water samples, Abstract;
Cadmium induced testicular pathophysiology: Prophylactic role of taurine: P. Sil, et al. ; Reprod. Toxicol. 26, 282 (2008), Application(s): EIA using mouse plasma, Abstract;
Effect of oral methyl-t-butyl ether (MTBE) on the male mouse reproductive tract and oxidative stress in liver: A. de Peyster, et al. ; Reprod. Toxicol. 26, 246 (2008), Application(s): EIA using mouse serum, Abstract;
Expression of arginine vasotocin in distinct preoptic regions is associated with dominant and subordinate behaviour in an African cichlid fish: R. Fernald, et al. ; Proc. Biol. Sci. 275, 2393 (2008), Application(s): EIA using fish plasma, Abstract;
Photoperiod and Testosterone Interact to Drive Seasonal Changes in Kisspeptin Expression in Siberian Hamsters (Phodopus sungorus): L. Kriegsfeld, et al. ; J. Neuroendocrinol. 20, 1339 (2008), Application(s): EIA using hamster serum, Abstract;
Adrenal hormones mediate melatonin-induced increases in aggression in male Siberian hamsters (Phodopus sungorus): G.E. Demas, et al.; Horm. Behav. 46, 582 (2004), Application(s): Blood serum testosterone and serum cortisol from Siberian hamsters, Abstract;
Behavioural and neuroendocrine adaptations to repeated stress during puberty in male golden hamsters: J.C. Wommack, et al. ; J. Neuroendocrinol. 16, 767 (2004), Application(s): EIA using hamster plasma, Abstract;
Infertility with defective spermatogenesis and hypotestosteronemia in male mice lacking the androgen receptor in Sertoli cells: C. Chang, et al. ; PNAS 101, 6876 (2004), Application(s): EIA using mouse serum, Abstract;
Effects of Medical or Surgical Castration on Erectile Function in an Animal Model: A.M. Traish, et al. ; J. Androl. 24, 381 (2003), Application(s): EIA using mouse cell lysates, Abstract;
In Utero and Lactational Exposure to 2,3,7,8-Tetrachlorodibenzo-p-dioxin in the C57BL/6J Mouse Prostate: Lobe-Specific Effects on Branching Morphogenesis: K. Ko, et al. ; Toxicol. Sci. 70, 227 (2002), Application(s): EIA using mouse tissue, Abstract; Full Text

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