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Substance P ELISA kit

Highly sensitive Substance P ELISA kit enabling detection of Substance P in plasma, serum, saliva, urine, and supernatants in just 3 hours.
ADI-901-018 5x96 wells 1,527.00 USD
Do you need bulk/larger quantities?
Alternative size available: ADI-900-018 (1x96 wells)
  • Highly sensitive assay, measuring as little as 8.04 pg/ml of Substance P
  • Widely cited in numerous peer reviewed publications
  • High throughput fomat, assay up to 38 samples in duplicate per 96 well kit
  • Fully quantitative results that surpass semi-quantitative Western blot analysis
  • Save with bulk kit package!
The Substance P EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 3 hours. Absorbance is read at 405 nm. This kit utilized low sample volume and provides the ability to assay up to 38 samples in duplicate per 96 well kit.

Product Specification

Sensitivity:8.04 pg/ml (range 9.76 - 10,000 pg/ml)
Assay Time:3 hours
Applications:ELISA, Colorimetric detection
Application Notes:For the quantitative determination of Substance P in culture supernatants, plasma, serum, saliva, and urine from any species. Cited sample types include tissue.
Species reactivity:Species independent
Use/Stability:Store all components at +4°C, except standard and conjugate at -20°C.
Shipping:Shipped on Blue Ice
Contents:GxR Microtiter plate, Conjugate, Antibody, Assay buffer concentrate, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution
Scientific Background:Substance P is an undecapeptide hormone (RPKPQQFFGLM) that mediates a number of biological activities including nausea, anxiety, pain, and vasodilation. It is a member of the tachykinin family of neuropeptides, and is widely distributed in the peripheral and central nervous systems of vertebrates.
UniProt ID:P20366

Product Literature References

Effects on breathing of agonists to μ-opioid or GABAA receptors dialyzed into the Ventral Respiratory Column of awake and sleeping goats: T.M. Langer, et al.; Respir. Physiol. Neurobiol. 239, 10 (2017), Abstract;
Flunixin meglumine improves pregnancy rate in embryo recipient beef cows with an excitable temperament: R.K. Kasimanickam, et al.; Theriogenology 107, 70 (2017), Abstract; Full Text
Acid-Sensing Ion Channel 1a Contributes to Airway Hyperreactivity in Mice: L.R. Reznikov, et al.; PLoS One 11, e0166089 (2016), Abstract; Full Text
Efficacy of oral meloxicam suspension for prevention of pain and inflammation following band and surgical castration in calves: M. Olson, et al.; BMC Vet. Res. 12, 102 (2016), Application(s): Measurment of substance P in blood samples, Abstract; Full Text
Evolution of Chronic Pain in Sickle Cell Disease: M. Pope, et al.; Blood 128, 1297 (2016), Full Text
Substance P Receptor Signaling Mediates Doxorubicin-Induced Cardiomyocyte Apoptosis Triple-Negative Breast Cancer Chemoresistance: P. Robinson, et al.; Biomed. Res. Int. 2016, Article ID 1959270 (2016), Application(s): Quantitation of SP Protein in cell lysates, Abstract; Full Text
The role of substance P in the maintenance of colonic hypermotility induced by repeated stress in rats: P. Lu, et al.; Neuropeptides 56, 75 (2016), Application(s): Determine levels of substance P in serum, Abstract;
Blockade of neurokinin-1 receptors in the ventral respiratory column does not affect breathing but alters neurochemical release: C. Muere, et al.; J. Appl. Physiol. 118, 732 (2015), Application(s): ELISA, Abstract;
Characteristics and Potential Biomarkers for Chronic Pain in Patients with Sickle Cell Disease: N. Kuei, et al.; Blood 126, 986 (2015), Application(s): Human Substance P levels were measured, Full Text
Effect of anti-NGF antibodies in a rat tibia fracture model of complex regional pain syndrome type I: J. Clark, et al. ; Pain 138, 47 (2008), Application(s): EIA using rat tissue (ref. 1), Abstract;

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