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cGMP ELISA kit

Most sensitive (0.088 pmol/ml) colorimetric kit  for extracellular cGMP quantification.
 
ADI-901-013 5x96 wells 1,414.00 USD
Do you need bulk/larger quantities?
 
Alternative size available: ADI-900-013 (1x96 wells)
  • Sensitive – optional acetylation protocol increases sensitivity 10-fold (0.088 pmol/ml)
  • Rapid – reliable results in just 3 hours
  • Trusted – regularly cited in peer-reviewed publications
  • Reduce errors – ready-to-use color-coded reagents and pre-coated plates
  • Simple – efficient and well-established sample handling protocols
  • Reliable – consistent lot-to-lot performance
  • Save with bulk kit package!
This is a colorimetric competitive immunoassay kit for the quantitative determination of extracellular cGMP. Sensitivity is increased by acetylation (reagents included). Absorbance is read at 405 nm.The ready-to-use liquid color-coded reagents provided reduce error.

Product Specification

Alternative Name:cyclic GMP
 
Sensitivity:(non-acetylated) 0.37 pmol/ml; (acetylated) 0.088 pmol/ml (range (non-acetylated) 0.16 - 500 pmol/ml; (acetylated) 0.16 - 100 pmol/ml)
 
Assay Time:3 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of cGMP in culture supernatants, plasma, serum, saliva, and urine from any species.
 
Species reactivity:Species independent
 
Crossreactivity:cyclic GMP (100%) and <0.001%: GMP, GTP, cyclic AMP, AMP, ATP, cyclic UMP, CTP
 
Use/Stability:Store all components at +4°C, except standard and conjugate at -20°C.
 
Shipping:Shipped on Blue Ice
 
Kit/Set Contains:GxR Microtiter plate, Conjugate, Antibody, Assay buffer 2 concentrate, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution, Acetic anhydride, Triethylamine
 
Scientific Background:Cyclic GMP (cGMP), which has been shown to be present at levels typically 10-100 fold lower than cAMP, is formed by the action of the enzyme guanylate cyclase on GTP. Some hormones, such as Acetylcholine, Insulin, and Oxytocin, as well as certain other chemicals like Serotonin and Histamine cause an increase in cGMP levels. Stimulators of guanylate cyclase such as the vasodilators nitroprusside, nitroglycerin, sodium nitrate, and nitric oxide (NO) also stimulate cGMP levels.
 

Product Literature References

Sildenafil attenuates hypoxic pulmonary remodelling by inhibiting bone marrow progenitor cells: S. Favre, et al.; J. Cell. Mol. Med. 21, 871 (2017), Abstract;
A Soluble Guanylate Cyclase Activator Inhibits the Progression of Diabetic Nephropathy in the ZSF1 rat: C.M. Boustany-Kari, et al.; J. Pharmacol. Exp. Ther. 356, 712 (2016), Application(s): Cyclic GMP levels were determined in frozen kidney cortex sections, Abstract; Full Text
Ginsenoside Re Enriched Fraction (GS-F3K1) from Ginseng Berries Ameliorates Ethanol-Induced Erectile Dysfunction via Nitric Oxide-cGMP Pathway: M.K. Pyo, et al.; Nat. Prod. Sci. 22, 46 (2016), Application(s): Measured the cGMP level in the corpus cavernosum tissue, Full Text
Implantation and pregnancy outcome of Sprague-Dawley rats fed with low and high salt diet: G.O. Oludare, et al.; Middle East Fertil. Soc. J. (2016), Application(s): Measured rat serum cGMP levels,
Platelet inhibitory effects of juices from Pachyrhizus erosus L. root and Psidium guajava L. fruit: a randomized controlled trial in healthy volunteers: T. Thaptimthong, et al.; BMC Complement. Altern. Med. 16, 269 (2016), Application(s): Determination of plasma cGMP concentrations, Abstract; Full Text
Quercetin Inhibits Pacemaker Potentials via Nitric Oxide/cGMP-Dependent Activation and TRPM7/ANO1 Channels in Cultured Interstitial Cells of Cajal from Mouse Small Intestine: H. Gim, et al.; Cell. Physiol. Biochem. 35, 2422 (2015), Application(s): ELISA using murine small intestine interstitial cells of Cajal (ICCs), Abstract; Full Text
The Influence of Nitric Oxide on Soluble Guanylate Cyclase Regulation by Nucleotides: the Role of Pseudosymmetric Site: N. B. Surmeli, et al.; J. Biol. Chem. 290, 15570 (2015), Application(s): ELISA, Abstract; Full Text

Related Products

cGMP ELISA kit 

Most sensitive (0.088 pmol/ml) colorimetric kit for extracellular cGMP quantification.
ELISA, Colorimetric detection | Print as PDF
 
ADI-900-013 96 wells 352.00 USD
Do you need bulk/larger quantities?
 

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