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17β-Estradiol ELISA kit

Highly sensitive ELISA, measuring as little as 28.5 pg/ml of 17β-Estradiol in < 3 hours
 
ADI-901-008 5x96 wells 1,072.00 USD
Do you need bulk/larger quantities?
 
Alternative size available: ADI-900-008 (1x96 wells)
  • Specific with low cross-reactivity to related steroids
  • Safe and non-radioactive enzymatic assay
  • Simple and color-coded reagents reduces error
  • Reliable and reproducible results day-after-day and lot-after-lot
  • Fully quantitative results that surpass semi-quantitative Western blot analysis
  • Save with bulk kit package!
The 17β-Estradiol EIA kit is a colorimetric competitive enzyme immunoassay kit with results in < 3 hours. Absorbance is read at 405 nm. This kit offers specific and save non-radioactive quantification of estradiol with ready-to-use liquid color-coded reagents to reduce error.
ADI-900-008 std curve
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ADI-900-008 std curve

Product Specification

Alternative Name:Estrogen
 
Sensitivity:28.5 pg/ml (range 29.3 - 30,000 pg/ml)
 
Assay Time:<3 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of 17β-Estradiol in culture supernatants and saliva from any species. For serum and plasma use ADI-901-174.
 
Species reactivity:Species independent
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Kit/Set Contains:GxR Microtiter plate, Conjugate, Antibody, Assay buffer concentrate, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution, Steroid displacement reagent
 
Scientific Background:Biologically active estrogen, 17β-estradiol, is an important sex hormone, but also makes an important contribution to the high concentrations of estrogens, which are present in malignant breast tissues.
 
Technical Info/Product Notes:For an overview of cited samples, please click here
 

Product Literature References

Estrogen activation of microglia underlies the sexually dimorphic differences in Nf1 optic glioma-induced retinal pathology: J.A. Toonen, et al.; J. Exp. Med. 214, 17 (2017), Application(s): ELISA for human samples, Abstract; Full Text
Effects of Supplementation of Human Endometriotic Fluids on In Vitro Mouse Preantral Follicle Culture: S.K. Kim, et al.; Reprod Sci. (2016), Abstract;
Implantation and pregnancy outcome of Sprague-Dawley rats fed with low and high salt diet: G.O. Oludare, et al.; Middle East Fertil. Soc. J. (2016), Application(s): Measured rat serum estradiol levels,
Optimizing multi-dimensional high throughput screening using zebrafish: L. Truong, et al.; Reprod. Toxicol. 65, 139 (2016), Application(s): Nominal exposure concentration measurement of 17β-estradiol and 17α-ethinylestradiol, Abstract;
Automated and portable solid phase extraction platform for immuno-detection of 17β-estradiol in water: S. Heub, et al.; J. Chromatogr. A. 1381, 22 (2015), Application(s): ELISA using water samples, Abstract;
Expression and activity of Rac1 is negatively affected in the dehydroepiandrosterone induced polycystic ovary of mouse: V. Maurya, et al.; J. Ovarian Res. 7, 32 (2014), Application(s): EIA using mouse ovarian tissue, Abstract; Full Text
Tumor endothelial marker 1–specific DNA vaccination targets tumor vasculature: J. Facciponte, et al.; J. Clin. Invest. 124, 1497 (2014), Application(s): EIA using mouse serum, Abstract; Full Text
Female preference for males depends on reproductive physiology in the African cichlid fish Astatotilapia burtoni: M.R. Kidd, et al.; Gen. Comp. Endocrinol. 180, 56 (2013), Application(s): ELISA using aquarium water, Abstract;
MEN1 is a melanoma tumor suppressor that preserves genomic integrity by stimulating transcription of genes that promote homologous recombination-directed DNA repair: M. Fang, et al.; Mol. Cell. Biol. 33, 2635 (2013), Application(s): ELISA using cell lysates, Abstract; Full Text
Preliminary findings of fecal gonadal hormone concentrations in six captive sea otters (Enhydra lutris) after deslorelin implantation: S. Larson, et al.; Zoo Biol. 32, 307 (2013), Application(s): EIA using sea otter feces, Abstract;
Sex steroid hormones modulate responses to social challenge and opportunity in males of the monogamous convict cichlid, Amatitliana nigrofasciata: A. Sessa, et al.; Gen. Comp. Endocrinol. 189, 59 (2013), Application(s): EIA using fish plasma, Abstract;
Fecal Hormones Measured within Giant Pacific Octopuses Enteroctopus dofleini: S. Larson & R. Anderson; J. Aquat. Anim. Health 22, 152 (2010), Application(s): EIA using octopus feces, Abstract;
Measuring multiple hormones from a single water sample using enzyme immunoassays: C. Kidd, et al.; Gen. Comp. Endocrinol. 165, 277 (2010), Application(s): EIA using fish plasma and water samples, Abstract;
Sex steroid-mediated reprogramming of vascular smooth muscle cells to stem cells and neurons: possible utilization of sex steroid combinations for regenerative treatment without utilization of in vitro developed stem cells: A. Bukovsky; Cell Cycle 8, 4079 (2009), Application(s): EIA using ovarian epithelial cells, Abstract;
Comparison of three enzyme immunoassays for measuring 17beta-estradiol in flushed dairy manure wastewater: T. Hanselman, et al.; J. Environ. Qual. 33, 1919 (2004), Application(s): EIA using livestock feces, water samples, Abstract;

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