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Fibrinogen (human) ELISA kit

Sensitive, easy-to-use ELISA kit to quantify human fibrinogen, a glycoprotein and essential component of the blood coagulation system.
 
ADI-900-230-0001 96 wells 561.00 USD
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  • Sensitive ELISA, detecting as little as 7.63 ng/ml of human fibrinogen
  • Flexible sample types, including: cell lysates, plasma, and serum
  • Simple protocol with results in just 3.5 hours
  • Quantitative results as compared to Western blot analysis
  • Low cross reactivity to similar molecules
The Fibrinogen (human) ELISA kit is a complete, colorimetric, immunometric immunoassay kit for the quantitative determination of human fibrinogen in cell lysate, plasma, and serum samples with results in about 3.5 hours.
ADI-900-230 std curve
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ADI-900-230 std curve

Product Specification

Sensitivity:<7.63 ng/ml (range 15.6 - 1000 ng/ml)
 
Assay Time:~3.5 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of human fibrinogen in cell lysate, plasma, and serum samples.
 
Species reactivity:Human
 
Crossreactivity:No cross reactivity to similar molecules (human serum albumin, transferrin, IgG, α-fetoprotein (AFP), plasminogen, vitamin D binding protein (VDBP), hemoglobin).
 
Handling:All kit components are stable at 2 to 8°C. Any unused reconstituted standard should be discarded or frozen at -70°C. Standard can be frozen and thawed one time only without loss of immunoreactivity.
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Kit/Set Contains:Microtiter Plate, Incubation Buffer, Washing Buffer, Protein Standard, Dilution Buffers, Secondary Antibody, AV-HRP, TMB Substrate, Stop Solution
 
Scientific Background:Fibrinogen is a soluble glycoprotein found in the plasma, with a molecular weight of 340kDa. It comprises of three pairs of non-identical polypeptide chains (alpha, 63.5kDa beta, 56kDa, and gamma, 47kDa chains) linked to each other by disulphide bonds. Low plasma fibrinogen concentrations are therefore associated with an increased risk of bleeding due to impaired primary and secondary hemostasis. Therefore Fibrinogen is an essential component of the blood coagulation system. Also it may play key roles in the process of atherosclerotic lesion formation, with subsequent effects on cardiovascular diseases. And increasing evidence from epidemiological studies suggests that elevated plasma fibrinogen levels are associated with an increased risk of ischaemic heart disease (IHD), stroke and other thromboembolism.
 
UniProt ID:P02671 (α chain), P02675 (β chain), P02679 (γ chain)
 

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