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5-Hydroxymethylcytosine DNA ELISA kit

Highly sensitive, rapid ELISA kit to quantify 5-hydroxymethylcytosine in DNA with results that closely correlate to mass spectrometry analysis.
ADI-900-225-0001 96 wells 495.00 USD
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  • Rapid - fully quantitative results in < 3 hours
  • Convenient – user-friendly alternative to mass spectrometry
  • Simple – efficient and well-established protocol
  • High-throughput – 96 well format ideal for global detection, tissue-specific quantitation, high-throughput compound screening, and more
The 5-Hydroxymethylcytosine DNA ELISA kit is a complete, colorimetric, immunometric immunoassay kit for the quantitative determination of 5-hydroxymethylcytosine in DNA  with results in just 3 hours.
ADI-900-225 std curve
ADI-900-225 schematic
ADI-900-225 MS-ELISA
DNA Mass Spectroscopy versus ELISA analysis: The 5-hmC DNA ELISA Kit (prod. no. ADI-900-225) quantifies 5-hmC in numerous DNA samples with close correlation to LC-MS/MS-MRM analysis. DNA samples include: brain, kidney, liver, thymus, human embryonic stem cells and two carcinoma samples.
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ADI-900-225 std curve ADI-900-225 schematic ADI-900-225 MS-ELISA

Product Specification

Alternative Name:5-hmC
Sensitivity:<0.02% 5-hydroxymethylcytosine DNA per 100ng input DNA (range 0.03-0.55% (100 ng/µl))
Assay Time:~3 hours
Applications:ELISA, Colorimetric detection
Application Notes:For the quantitative determination of species independent 5-hydroxymethylcytosine DNA in a variety of DNA samples.
Species reactivity:Species independent
Use/Stability:Antibody and control set should be stored at -20°C, while the DNA HRP antibody should be stored at -80°C.
Shipping:Shipped on Blue Ice
Long Term Storage:+4°C
Contents:Microtiter Plate, Coating Buffer, ELISA Buffer, Primary Antibody, Antibody-conjugate,  HRP Developer, Controls
Scientific Background:The 5-methylcytosine (5-mC) modification of DNA in epigenetic regulation has been well studied over the last several decades. However, the role of the so-called “sixth base”, 5-hydroxymethylcytosine (5-hmC), has yet to be defined. Recent studies have associated 5-hmC patterns to transcriptional regulation of genes, which may contribute to normal and disease states of organisms.

General Literature References

Alterations of the lung methylome in allergic airway hyper-responsiveness: R.Y. Cheng, et al.; Environ. Mol. Mutagen. 55, 244 (2014), Abstract;

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5-Methylcytosine DNA ELISA kit 

Streamlined, rapid ELISA kit to quantify 5-methylcytosine in DNA with results that closely correlate to mass spectrometry analysis.
ELISA, Colorimetric detection | Print as PDF
ADI-900-224-0001 96 wells 495.00 USD
Do you need bulk/larger quantities?

5-Hydroxymethylcytosine polyclonal antibody 

ELISA, IP, Immunoblot | Print as PDF
ADI-905-904-0050 50 µg 218.00 USD
ADI-905-904-0200 200 µg 634.00 USD
Do you need bulk/larger quantities?

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