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5-Methylcytosine DNA ELISA kit

Streamlined, rapid ELISA kit to quantify 5-methylcytosine in DNA with results that closely correlate to mass spectrometry analysis.
 
ADI-900-224-0001 96 wells 472.00 USD
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  • Rapid – fully quantitative results in < 3 hours
  • Convenient – user-friendly alternative to mass spectrometry
  • Simple – efficient and well-established protocol
  • High-throughput – 96 well format ideal for global 5-methylcytosine detection in DNA
The 5-Methylcytosine DNA ELISA kit is a complete, colorimetric, indirect immunoassay kit for the quantitative determination of 5-methylcytosine in DNA with results in less than 3 hours.
ADI-900-224 std curve
ADI-900-224 schematic
ADI-900-224 Genomic DNA
Genomic DNA Mass Spectroscopy versus ELISA analysis: The 5-mC DNA ELISA Kit (prod. no. ADI-900-224) quantifies 5-mC in numerous DNA samples with close correlation to LC-MS/MS-MRM analysis. Genomic DNA samples include: human brain (HB), human kidney (HK), human embryonic stem cell (HESC), mouse brain (MB), mouse kidney (MK), and mouse testes (MT).
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ADI-900-224 std curve ADI-900-224 schematic ADI-900-224 Genomic DNA

Product Specification

Alternative Name:5-mC
 
Sensitivity:~0.5% 5-methylcytosine per 100ng single-stranded DNA (range 5-100% (100 ng/µl))
 
Assay Time:<3 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of species independent 5-methylcytosine DNA in a variety of DNA samples.
 
Species reactivity:Species independent
 
Use/Stability:Antibody, conjugate, and controls should be stored at -20°C
 
Handling:Store Box 1 at 4°C and Box 2 at -20°C.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:+4°C
 
Kit/Set Contains:Microtiter Plate, Coating Buffer, ELISA Buffer, Primary Antibody, Antibody-conjugate,  HRP Developer, Controls
 
Scientific Background:The ability to efficiently detect and quantify DNA methylation (i.e., 5-methylcytosine) has become essential for epigenetic-based research.  To date, a number of methods have been developed for this purpose including high-performance capillary electrophoresis, bisulfite sequencing, and methylated DNA immunoprecipitation.
 

General Literature References

Nutri-epigenetics ameliorates blood-brain barrier damage and neurodegeneration in hyperhomocysteinemia: role of folic acid: A. Kalani, et al.; J. Mol. Neurosci. 52, 202 (2013), Abstract; Full Text
Temozolomide downregulates P-glycoprotein expression in glioblastoma stem cells by interfering with the Wnt3a/glycogen synthase-3 kinase/β-catenin pathway: C. Riganti, et al.; Neuro. Oncol. 15, 1502 (2013), Abstract;

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Do you need bulk/larger quantities?
 

5-Methylcytosine monoclonal antibody (10G4) 

ELISA, IP, Immunoblot | Print as PDF
 
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5-Hydroxymethylcytosine polyclonal antibody 

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