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Angiotensin A ELISA kit

Most sensitive Angiotensin A kit for cardiovascular research.
 
ADI-900-207 96 wells Inquire for pricing
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Minimum Order Quantity: 5
  • Ultra-Sensitive - detect as little as 0.2pg/ml of Angiotensin A
  • Rapid - results in just 3 hours from up to 40 samples in duplicate
  • Broad dynamic range - easily quantify high and low levels of Angiotensin A
  • Easy-to-use - liquid color-coded reagents reduce errors
The Angiotensin A EIA kit is a colorimetric, competitive immunoassay kit with results in just 3 hours.
900-207 parallelism
900-207 typical data
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900-207 parallelism 900-207 typical data

Product Specification

Sensitivity:8.3 pg/ml (range 9.8 - 10,000 pg/ml)
 
Assay Time:3 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of species independent Angiotensin A in plasma and serum samples.
 
Species reactivity:Species independent
 
Crossreactivity:Angiotensin A (100%), Angiotensin (1-12) (<0.5%)
 
Use/Stability:Store all components at +4ºC, except Standard at -20ºC.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Kit/Set Contains:Microtiter Plate, Conjugate, Antibody, Assay Buffer 16, Wash Buffer Concentrate, Standard, TMB Substrate, Stop Solution 2, Streptavidin-HRP
 
Scientific Background:Angiotensins are small peptides derived from angiotensinogen. Several of the known angiotensins are established endocrine effectors in the regulation of blood pressure, but they are also known to have other functions locally, in several organs and tissues (paracrine) and at the cellular level (autocrine / intracrine). Angiotensin I (DRVY-IHPFHL) has no known effector function but it is an immediate precursor of Angiotensin II (DRVYIHPF). Angiotensin II binds AT1 receptors, which promote vasoconstriction, sodium retention, release of aldosterone, release of Arg-vasopressin, cell proliferation, inflammation, fibrosis, anxiety, and cardiac hypertrophy. Angiotensin A (ARVYIHPF), and Angiotensin III (RVYIHPF), also bind AT1 receptors. Angiotensin (1-7) (DRVYIHP) binds a dif-ferent receptor called MAS-1 which has opposite effects (vasodilation, natriuresis, anti-proliferation, NO release, PGE release, and apoptosis). Angiotensin IV (VYIHPF) binds yet another receptor called AT4 (IRAP), which promotes increase of blood flow, angiogenesis, and natriuresis, and which has also been implicated in memory formation and in the pathogenesis of Alzheimer’s disease. The peptide LVV-hemorphin-7 (LVVYPWTQRF), which is not an angiotensin, also binds the AT4 receptor. There are other angiotensin peptides that have been identified, including Ang (1-9) (DRVYIHPFH), Ang (1-12) (DRVY-IHPFHLVI), Ang V (3-7) (VYIHP), as well as several other shorter peptides that have undetermined functions.
 
UniProt ID:P01019 (human)
 

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