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Sensitive - reliably measure as little as 1.7pg/ml of IL-33
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Low dynamic range provides sensitive measurements
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Specific - low cross-reactivity to other interleukins
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Non-radioactive - enzymatic assay format is safe and reliable
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Easy-to-use - liquid color-coded reagents reduce errors
The IL-33 (human), EIA kit is a colorimetric immunometric immunoassay kit with results in 3 hours.
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Product Details
| Alternative Name: | Interleukin-33, NF-HEV |
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| Sensitivity: | 1.7 pg/ml (range 7.8 - 500 pg/ml) |
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| Assay Time: | 3 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of human IL-33 in cell lysates, culture supernatants, serum, plasma and synovial fluid samples. |
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| Wavelength: | 450 nm |
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| Species reactivity: | Human
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| Crossreactivity: | Human IL-33 (100%), mouse IL-33 (<0.02%). Unaffected by human IL-1α, IL-2, IL-10, MCP-1, IL-13, MIP-1β, IL-4, IL-6, IL-8, TNFα, IFNγ, IL-1β IL-5, IL-15, IL-17, IL-21, IL-29, RANTES |
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| Quantity: | 1 x 96-well plate |
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| Use/Stability: | Store all components at +4ºC, except Standard at -20ºC. |
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| Shipping: | Blue Ice |
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| Short Term Storage: | -20°C |
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| Long Term Storage: | -20°C |
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| Contents: | Microtiter Plate, Conjugate, Antibody, Assay Buffer 13, Wash Buffer Concentrate, Standard, TMB Substrate, Stop Solution 2, Extraction Reagent Concentrate |
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| Scientific Background: | IL-33, also named Nuclear Factor from High Endothelial Venules (NF-HEV), is a member of the Interleukin-1 (IL-1) family. Members of this family, such as IL-1α/β and IL-18, are known to play important roles in host defense, immune regulation, neuronal injury and inflammation. IL-33 has been reported to be the specific ligand for ST24, an orphan receptor of the IL-1 receptor family. Stimulation of ST2 by IL-33 induces NF-κB and MAPK signaling pathways. IL-33 is synthesized in response to cytokines such as IL-1β and TNF-α and plays an important role in TH2-associated immunology by triggering increased secretion of IL-5 and IL-13 from polarized TH2 cells. IL-33 has also been found to be localized in the nucleus of human epithelial and endothelial cells, and can be present in serum and synovial fluid samples of patients with rheumatoid arthritis. Overexpression of IL-33 in mice has been found to induce asthmatic symptoms, and enhance joint inflammation. |
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| Technical Info/Product Notes: | Cited samples:
Cytokine ELISAs Cited Samples |
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| UniProt ID: | O95760 |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Genetic variant in IL-33 is associated with idiopathic recurrent miscarriage in Chinese Han population: J. Yue, et al.; Sci. Rep.
6, 23806 (2016),
Application(s): Measured serum IL-33,
Abstract;
Full Text
Nuclear IL-33 regulates soluble ST2 receptor and IL-6 expression in primary human arterial endothelial cells and is decreased in idiopathic pulmonary arterial hypertension: D. Shao, et al.; Biochem. Biophys. Res. Commun.
451, 8 (2014),
Application(s): Human serum and cell culture supernantant,
Abstract;
Interleukin 33 as a mechanically responsive cytokine secreted by living cells: R. Kakkar, et al.; J. Biol. Chem.
287, 6941 (2012),
Abstract;
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