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Serotonin ELISA kit

Easy-to-use, well-validated serotonin ELISA kit for hormone research.
 
ADI-900-175 96 wells 530.00 USD
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  • Highly sensitive measurement of serotonin, detecting as little as 0.293 ng/ml
  • No acylation required for standards and samples
  • Thoroughly validated in multiple complex sample matrices
  • High throughput format with results in just 3 hours for up to 39 samples in duplicate
  • Fully quantitative results that surpass semi-quantitiave Western blot analysis
The Serotonin EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 3 hours.
ADI-900-175 std curve
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ADI-900-175 std curve

Product Specification

Alternative Name:5-HT, 5-hydroxytryptamine
 
Sensitivity:0.293 ng/ml (range 0.49 - 500 ng/ml)
 
Assay Time:3 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of Serotonin in platelets, plasma, serum, and urine from any species. Cited sample type includes culture supernatant.
 
Species reactivity:Species independent
 
Crossreactivity:N-acetyl serotonin (17%), 5-hydroxy-L-tryptophan (0.4%), Tryptamine (0.1%), 5-hydroxyindoleacetic acid (0.03%), Melatonin (0.01%), Tyramine (<0.004%), Tryptophan (<0.004%)
 
Use/Stability:Store all components at -20°C
 
Shipping:Shipped on Blue Ice
 
Kit/Set Contains:GxR IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution
 
Scientific Background:Serotonin (5-hydroxytryptamine, 5-HT) is a monoamine found in the central nervous system, gastrointestinal tract, and blood with broad physiological functions in neurotransmission, gastric motility, hemostasis, and cardiovascular integrity. Seven subclasses (5-HT 1-7) of serotonin receptors have been identified, a majority of which are members of the seven transmembrane domain-containing GPCR family, and are the target of several anti-depressant drugs.  The 5-HT1 subclass consists of five highly homologous receptors that couple via G(i/o) alpha subunits to inhibit adenylyl cyclase activity. 5-HT1A expression in mammals is strongest in CNS limbic pathways that control emotion, including tissue of the hippocampus, dorsal raphé, and septum.
 

Product Literature References

Brain injury induces specific changes in the caecal microbiota of mice via altered autonomic activity and mucoprotein production: A. Houlden, et al.; Brain. Behav. Immun. 57, 10 (2016), Application(s): Mouse tissue serotonin levels were measured, Abstract;
Cardiac volume overload and pulmonary hypertension in long-term follow-up of patients with a transjugular intrahepatic portosystemic shunt: A. Wannhoff, et al.; Aliment. Pharmacol. Ther. 43, 955 (2016), Application(s): Measured peripheral blood levels of serotonin, Abstract; Full Text
Generation of functional human serotonergic neurons from fibroblasts: K.C. Vadodaria, et al.; Mol. Psychiatry 21, 49 (2016), Application(s): Serotonin levels in culture supernatants, Abstract;
Heightened aggressive behavior in mice deficient in aldo-keto reductase 1a (Akr1a): T. Homma, et al.; Behav. Brain Res. 22, 319 (2016), Application(s): Plasma serotonin levels measured in mice, Abstract;
M30 Antagonizes Indoleamine 2,3-Dioxygenase Activation and Neurodegeneration Induced by Corticosterone in the Hippocampus: C.S. Lam, et al.; PLoS One 11, e0166966 (2016), Application(s): Serotinin levels in the hippocampal tissue lysate were measured , Abstract; Full Text
Serotonin Activates Bacterial Quorum Sensing and Enhances the Virulence of Pseudomonas aeruginosa in the Host: L.D. Knecht, et al.; EBioMedicine 9, 161 (2016), Application(s): Serotonin determination in mouse intestinal homogenates and stool samples, Abstract;
Suicide, Impulsivity and its Relationship to Platelet Serotonin Levels: A. Kumar, et al.; IJCMR 3, 3077 (2016), Application(s): Serotonin levels in Human platelets were measured, Full Text
Unveiling the interactions among BMPR-2, ALK-1 and 5-HTT genes in the pathophysiology of HAPE: Z. Ali, et al.; Gene 588, 163 (2016), Application(s): Measured plasma 5-HT levels, Abstract;
Use of the RatLoft decreases pup mortality in lactating mice: S.R. Weaver, et al.; Lab. Anim. 50, 370 (2016), Application(s): Serotonin levels in mouse serum, Abstract;
Altered serotonin (5-HT) 1D and 2A receptor expression may contribute to defective insulin and glucagon secretion in human type 2 diabetes: H. Bennet, et al.; Peptides 71, 113 (2015), Application(s): ELISA using human islets, Abstract;
Elevated levels of 14-3-3 proteins, serotonin, gamma enolase and pyruvate kinase identified in clinical samples from patients diagnosed with colorectal cancer: P. Dowling, et al.; Clin. Chim. Acta 441, 133 (2015), Application(s): EIA using human plasma samples, Abstract;
Human tear serotonin levels correlate with symptoms and signs of dry eye: P. Chhadva, et al.; Ophthalmology 122, 1675 (2015), Application(s): Serotonin levels in human tears, Abstract;
Supplement of 5-hydroxytryptophan before induction suppresses inflammation and collagen-induced arthritis: T.H. Yang, et al.; Arthritis Res. Ther. 17, 364 (2015), Application(s): Serotonin levels in mouse serum, Abstract; Full Text
Peripheral Serotonin Regulates Maternal Calcium Trafficking in Mammary Epithelial Cells during Lactation in Mice: J. Laporta, et al.; PLoS One 9, e110190 (2014), Application(s): EIA using mouse serum, Abstract; Full Text
Serotonin regulates calcium homeostasis in lactation by epigenetic activation of hedgehog signaling: J. Laporta, et al.; Mol. Endocrinol. 28, 1866 (2014), Application(s): EIA using mouse serum, Abstract;
Feeding 5-hydroxy-l-tryptophan during the transition from pregnancy to lactation increases calcium mobilization from bone in rats: J. Laporta, et al.; Domest. Anim. Endocrinol. 44, 176 (2013), Application(s): EIA using rat serum, Abstract;
Lactobacillus johnsonii inhibits indoleamine 2,3-dioxygenase and alters tryptophan metabolite levels in BioBreeding rats: R. Valladares, et al.; FASEB J. 27, 1711 (2013), Application(s): EIA using rat serum, Abstract;
Serotonin (5-HT) affects expression of liver metabolic enzymes and mammary gland glucose transporters during the transition from pregnancy to lactation: J. Laporta, et al.; PLoS One 8, e57847 (2013), Application(s): EIA using rat serum, Abstract; Full Text
Short communication: Circulating serotonin (5-HT) concentrations on day 1 of lactation as a potential predictor of transition-related disorders: J. Laporta, et al.; J. Dairy Sci. 96, 5146 (2013), Application(s): EIA using bovine serum and plasma, Abstract;
Local serotonin mediates cyclic strain-induced phenotype transformation, matrix degradation, and glycosaminoglycan synthesis in cultured sheep mitral valves: C.M. Lacerda, et al.; Am. J. Physiol. Heart Circ. Physiol. 302, H1983 (2012), Application(s): EIA using media of sheep mitral valves, Abstract;
Perinatal growth restriction is not related to higher intestinal distribution and increased serum levels of 5-hydroxytryptamin in piglets: S. Willemen, et al.; J. Anim. Sci. 90S4, 305 (2012), Application(s): EIA using piglet serum, Abstract;
Static and cyclic tensile strain induce myxomatous effector proteins and serotonin in canine mitral valves: C.M. Lacerda, et al.; J. Vet. Cardiol. 14, 223 (2012), Application(s): EIA using media of canine mitral valves, Abstract;
Tryptophan hydroxylase-1 regulates immune tolerance and inflammation: E.C. Nowak, et al.; J. Exp. Med. 209, 2127 (2012), Application(s): EIA using mouse serum, Abstract; Full Text

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